Interleukin-1¦Â And Radix Astragali On Airway Epithelial Cells Of Insulin-like Growth Factor 1 Expression

Objective: To investigate the effect of IL-1β and Astraglus in expression of insulin –like growth factor1 in cultured rabbit airway epithelia cells. Methods: The cultured airway epithelia cells in vivo were randomly divided into treatment group and normal control group, To complete this experiment , we need three steps.(1) The expression of IGF-1 protein after airway epithelia cells were treated with IL-1β(1 ng/ml ) at different time: Treatment group were treated with IL-1β1 ng/ml, normal control group were treated with equal culture medium, supernatant and cell-coated dishes were attained after 4h,8h,16h﹑24h,48h respectively.(2) The expression of IGF-1 protein after airway epithelia cells were treated with IL-1β(different concentrition): Treatment group(IL-1β0.05ng﹑0.1 ng,1 ng,10 ng group)were treated with IL-1β0.05ng/ml﹑0.1 ng/ml,1 ng/ml,10 ng/ml respectively, normal control group were treated with equal culture medium. supernatant and cell-coated dishes were attained after 16h.(3) The expression of IGF-1 protein after airway epithelia cells were treated with IL-1β(10 ng/ml ) and Astraglus (different concentrition) together: Treatment group( Astraglus 50 mg,200 mg,500 mg group) were treated with IL-1β(10 ng/ml) and Astraglus 50 mg/ml,200 mg/ml,500 mg/ml respectively, control group were treated with IL-1β10 ng/ml, their culture medium were equal, supernatant and cell-coated dishes were attained after 16h. The expression of IGF-1 protein was measured by ELISA and immunocytochemistry.Results:(1) The level of IGF-1 at 16h﹑24h﹑and 48h in treatment group (0.5774±0.0357,0.5373±0.0412,0.5268±0.0670; 0.1407±0.0168, 0.1137±0.0322, 0.1073±0.0266 )were significantly higher than those from normal control group(0.2599±0.0026, 0.2601±0.0124,0.2669±0.0043；0.0545±0.0141,0.0533±0.0046,0.0562±0.0091)(p﹤0.05,respectively), but the level of IGF-1 at 4h﹑8h in treatment group were not different from those at 4h,8h in normal control group. (2) The level of IGF-1 in the IL-1β0.1 ng/ml group,1 ng/ml group and 10 ng/ml group (0.349±0.015,0.473±0.020,0.566±0.030 ;0.086±0.021,0.114±0.019,0.142±0.017) were significantly higher than those from normal control group(0.261±0.016；0.054±0.003 )and IL-1? 0.05ng/ml group(0.282±0.026；0.055±0.038),(p﹤0.05,respectively). The level of IGF-1 was significantly higher in the IL-1β10 ng/ml group than those from IL-1β0.05ng/ml group,IL-1β0.1ng/ml group and IL-1β1ng/ml group(p﹤0.05,respectively). (3) the concentration of IGF-1 from Astraglus 50mg,200mg,500mg group(0.4553±0.051,0.3959±0.083,0.2938±0.074;0.103±0.011,0.085±0.009,0.073±0.009) were much more lower than IL-1β10 ng/ml group(0.5659±0.030；0.142±0.167 ),(p﹤0.05,respectively), the concentration of IGF-1 from Astraglus 500mg group were significantly lower than Astraglus 50mg group and IL-1β10 ng/ml group(p﹤0.05,respectively),and were not different from Astraglus 200mg group(p﹥0.05). Conclusions: It was suggested that IL-1βmight accelerate airway remodeling occur and develop by increasing the IGF-1 expression and that Astraglus might prevent airway remodeling by decreasing the IGF-1 expression, which may contribute to its antiinflammatory effect.