Three Baoxin On Myocardial Ischemia - Reperfusion Injury In Experimental Research

In many developed and developing countries, cardiovascular diseases becomes a key reason to people's death. It is of great importance to prevent and cure the coronary heart disease.Early reperfusion has clearly been shown to be the most effective means to prevent cell death after coronary artery occlusion. Thrombolytic or angioplastic therapy is now widely practiced in acute myocardial infarction patients. However, reperfusion of the ischemic myocardium may not always be completely beneficial, and there is new evidence that it may initiate a cascade of events that partially counteract the beneficial effects of blood flow restoration. This phenomenon has been termed " ischemia-reperfusion injury".The concept of "ischemia-reperfusion injury" encompasses a list of events including: reperfusion arrhythmias, micro-vascular damage, reversible myocardial mechanical dysfunction, and cell death which due to apoptosis or necrosis. Oxidative stress, intracellular calcium overload, and neutrophil activation have all been proposed to explain the pathogenesis and the functional consequences of the injury in the ischemic-reperfusion myocardium. The development of effective prevention and/or treatment strategies has gradually became the most urgent challenge to researchers.Looking for some herbal preparations with satisfied curative effects is a hotspot at present. It is well documented that a great deal of Chinese traditional medicine can protect the heart exposed to ischemia-reperfusion and the effects are multi-targets. The TCM compound SANBAOXIN comes from Li-lianda, the academician of China Academy of TCM, this compound is composed of the Salviae Miltiorrhizae, Epimedium, etc. Through the experiments such as myocardial ischemia, blood stream dynamics, blood rheology and platelet conglomeration, it is proved that SANXINBAO can improve the heart function, cure myocardial ischemia, regulate the blood rheology and anti-platelet-conglomeration.The study was to investigate the effects of SANBAOXIN on injury induced by myocardical ischemia-reperfusion (MIRI) and against vivo and vitro thrombosis in rats, and explore the possible mechanism. The experimental model of MIRI was established by ligation of left anterior descending coronary artery for 40min followed by reperfusion for 180min. Then observe the effects of SANBAOXIN on the area of myocardial infract, the amount of superoxide dismutase (SOD), malondialdehyde (MDA) and that of nitric oxide (NO). Apply the way of chandler to form vitro thrombosis and the way of electricity stimulation to form vivo thrombosis, observe the effects of SANBAOXIN against thrombosis.The results indicated that the high and middle dosage of SANBAOXIN could evidently decrease the area of myocardial infract(vs model group, P<0.05, P<0.01), and the content of MDA, obviously elevated the activity of SOD, and increased the amount of NO at the same time. The 10g.kg-l dosage of SANBAOXIN could lengthen the OT in a large scope(p<0.05). All SANBAOXIN dosages could inhibit the formation of vitro thrombosis (P<0.05; P<0.01) .In conclusion: SANBAOXIN has protective effects on myocardical ischemic reperfusion injury. The protective effects may be that it can dilate vessels, increase the activity of clearence enzyme of free radical and inhibit lipid peroxidation and inhibit the formation of vitro and vivo thrombus.