Effect Of Leptin Pretreatment On MPO、MDA And SOD Levels In Mice With Myocardial Ischemia Reperfusion Injury

Objective:To monitor the changes of myeloperoxidase(MPO)、 malondialdehyde(MDA) and superoxide dismutase(SOD) levels in the process of myocardial ischemia reperfusion injury(MIRI) in mice.Methods:Twenty male C57BL/6mice were randomized into4group:(1)The sham-operation group;(2)The myocardial ischemia reperfusion group (MIRI group), established by blocking left coronary artery anterior descending branch for30min followed by reperfusion;(3)The leptin pretreatment group (Leptin+MIRI group), established by injecting intraperitoneally leptin at30min before ischemia;(4)The leptin post-treatment group(MIRI+Leptin group); established by injecting intraperitoneally leptin at30min after reperfusion. Blood and heart samples were harvested from each mouse at6hours after reperfusion.We then analyzed the samples for myeloperoxidase(MPO)、 malondialdehyde(MDA)、superoxide dismutase(SOD) levels and histological analysis.Results:Administration of leptin before MIRI significantly decreased myeloperoxidase(MPO)、malondialdehyde(MDA) levels and averaged up superoxide dismutase(SOD)activity. Conclusions:Leptin pretreatment can reduced the myeloperoxidase (MPO)、malondialdehyde(MDA) and superoxide dismutase(SOD) expressions,and significantly reduced tissue death in cardiac mucle、hemorrhage degree,and reduce the accumulation of the neutrophils.