Early Rheumatoid Arthritis Patients Of Cold And Heat Syndromes Taxonomy Of Basic Research

AimsTo evaluate the relationship among the heat and cold syndrome, syndrome factors and the expression of the cytokine (TNF-α, IL-10, IL-8), clinical inflammatory indexes (ESR, CRP), clinical immune indexes (IgA, IgM, IgG, RF, C3) in female patients with rheumatoid arthritis of early stage using multiple statistical methods.MethodsThe blood samples were collected and detected for the following indexes such as cytokines (TNF-a, IL-10, IL-8), clinical inflammatory indexes (ESR, CRP), clinical immune indexes (IgA, IgM, IgG, RF, C3) from seventy female patients with rheumatoid arthritis at a early stage. The symptoms about syndrome and those indexes inferred above of the RA patients were collected and organized as the questionnaire of "Questionnaire of Clinical syndrome messages of RA patients " which were completed by the clinical doctors.The relationship between the syndrome and the expression of the cytokine (TNF- α, IL-10, IL-8), inflammatory indexes (ESR, CRP), immune indexes (IgA, IgM, IgG, RF, C3) in female patients with rheumatoid arthritis of early stage were analyzed by the integration of three kinds of statistical methods which included two-way classification ANOVA, principal components analysis and factor analysis and canonical correlation analysis using SPSS 12.0 and SAS 8.0 statistical software.Results1 Relationship of syndrome and three cytokines (TNF-a, IL-10, IL-8) in female patients with RA at an early stageThe two-way classification ANOVA didn't show that there were some relationship between heat and cold syndrome and the expression of these three cytokines. The same results were observed about the relationship of syndrome factors and these three cytokines using canonical correlation analysis.2 Relationship of syndrome and clinical inflammatory indexes (ESR, CRP) in female patients with RA at an early stageFrom the data analysis, we found that there were no significant difference of the value of ESR between heat and cold syndrome, and there were no association of the value of ESR among five syndrome factors.For CRP, we observed that the heat syndrome have higher value of CRP than cold syndrome. It was consistent with the results of canonical correlation analysis of five syndrome factors and CRP, which said that with the increase of CRP, the grade of heat syndrome factor improved too, but the grade of three other syndrome factor decreased.3 Relationship of syndrome and immune indexes (IgA, IgG, IgM, RF, C3) in female patients with RA at an early stageThe two-way classification ANOVA didn't show that there were some relationship between heat and cold syndrome and immune indexes (IgA, IgG, IgM, RF, C3). Canonical correlation analysis showed that there were no association between syndrome factors and immune indexes (IgA, IgG, IgM, RF, C3) in female patients with RA at an early stage, and so is the relationship of the heat and cold syndrome and the five immune indexes.ConclusionBased on the above data analysis, we can't see that in female patients with rheumatoid arthritis of early stage the cytokine (TNF-cc, IL-10, IL-8), clinical inflammatory indexes (ESR), immune indexes (IgA, IgG, IgM, RF, C3) have some association with the heat and cold syndrome. However, heat...