Exogenous Vegf Protective Effect On Young Rats With Hypoxic-ischemic Brain Injury

Objective : To investigate the effect of exogenous vasscular endothelialgrowth factor (VEGF) on apotosis and c-fos gene expression in brain neurons of 14-day-old rats after hypoxia-ischemia brain damage (HIBD). Method: To establish hypoxic-ischemic encephalopathy (HIE) medol by adopting Rice techinique.Tewenty-six 14-day-old SD rats were randomly assigned to sham group (n=6),control group (n=10),VEGF group (n=10).The VEGF group were given rhVEGF165(0.25mg/kg) by intravenous bolus injection.The control group were given the same volume of Normal Saline intravenous bolus injection.Using c-fos gene immunohistochemical method and H-E staining to observe the amount of apoptotic neurons and the expression of c-fos gene. Result: There were no apototic neurons and no c-fos gene expression in sham group.The amount of apototic neurons were decreased (p<0.01). And the expression of c-fos gene was declined inVEGF group compared with that of control group. Conclusion: Exogenous VEGF could reduce HIBD.And inhibit c-fos gene expression by intravenous bolus injection. It suggested that exogenous VEGF could play a protection role for juvenile HIE rats.