Quercetin, Pns Atherosclerotic Rat Aortic Pdgf-b Chain Mrna Expression

Object:To investigate the anti-atherosclerotic mechanism, the effect of quercetin and total panax notoginseng saponins and two different proportional combinations of them on expression of platelet-derived growth factor B chain mRNA in atherosclerotic rat's aorta was studied. Methods:sixty-five male Wistar rats were randomly distributed into 7 groups.One group was fed with standard fodder,and the others were fed with high lipid-diet for 12 weeks.After the rat models are made,rats of different drug-intervening groups were given quercetin,total panax notoginseng saponins or 2:1,3:1 two different proportional combinations of them for 4 weeks by gastrogavage respectively,and rats of drug-contrasting group were given ligustrazine for 4 weeks by gastrogavage.Finally, serum total cholesterol(TC), triglycerides(TG),low-density lipoprotein(LDL) and high-density lipoprotein (HDL) were determined by enzymology assay,serum C-reactive(CRP) were determined by immune nephelometery and the expression of platelet-derived growth factor B chain(PDGF-B) mRNA in atherosclerotic rat's aorta was determined by reverse transcription polymerase chain reaction(RT-PCR). Results:1.High lipid diet could induce atherosclerosis in rats. Comparing with control group,the level of blood lipids,CRP and the expression of PDGF-BmRNA of model group were increased in statistics(p<0.05).2.After treating with drugs,comparing with model group,the level of blood lipids,CRP and the expression of PDGF-BmRNA of four therapy groups were all decreased significantly in statistics(p<0.05).3.Comparing with other therapy groups, the level of LDL, CRP and the expression of PDGF-BmRNA of 3:1 therapy group which was the best efficacious group were all decreased significantly in statistics(p<0.05).4.Comparing with ligustrazine therapy group, the level of blood lipids,CRP and the expression of PDGF-B mRNA of 3:1 therapy group that was the best efficacious group was reduced significantly in statistics(p<0.05). Conclusions: Qi-Benefiting and Blood-Activating Chinese medical herbs quercetin ,total panax notoginseng saponins and 2:1,3:1 two different proportional combinations of them all can reduce the level of blood lipids and CRP,down-regulate the expression of PDGF-BmRNA.Application of combining quercetin and total panax notoginseng saponins has better effect,and meanmile,with the deal of Qi-Benefiting drug increasing,the function of anti-atherosclerosis tensifies.It has play a role in anti-atherosclerosis through reducing the level of blood lipids and CRP,down-regulating the expression of PDGF-BmRNA.