Yiqi Tongmai Oral Liquid On Myocardial Ischemia Reperfusion Injury In Cardiac Myocyte Apoptosis Gene

Objective: To study the effects of Yi Qi Tong Mai Oral Liquid on myocardial apoptosis induced by ischemia-reperfusion(I/R).Methods: The left anterior descene(LAD) coronary artery was ligated to establish the ischemia-reperfusion(I/R) heart model in rat. Immunohistochemical staining was used to detect the changes of apoptosis and protein expression of caspase-3, bcl-2, bax and fas genes. Histopathological changes in myocardium was also observed.Result: (1) The number and size of cardiomyocyte cell reduced after ischemia-reperfusion and increased by Yi Qi Tong Mai Oral Liquid. (2) The expression of caspase-3 gene increased by ischemia-reperfusion and decreased by Yi Qi Tong Mai Oral Liquid. The effect changes with the dose. (3) The expression of bcl-2 gene decreased by ischemia-reperfusion and increased by Yi Qi Tong Mai Oral Liquid. The medicine effect changes with the deferent dose. (4) The expression of bax and fas gene inregulating after ischemia-reperfusion and downregulating after Yi Qi Tong Mai Oral Liquid. The medicine effect changes with the deferent dose.Conclusions: Yi Qi Tong Mai Oral Liquid had obviously preventive and therapeutic effects on reperfusion injury and could inhibit cardiomyocyte apoptosis. The mechanism might be that bcl-2 gene expression was increased, caspase-3 gene,bax gene and fas gene were decreased following ischemia and reperfusion.