Electro Focal Cerebral Ischemia And Reperfusion In Rat Brain Microvascular Structure

Objective:Cerebral Vascular Disease (CVD) is one of the three disease threatening human health badly, which has the characteristic of high attack rate, high disabling rate and high fatal rate, it brings heavy burden to individual, family and society.Along with the old aged rapidness, the attack rate of cerabral vascular disease increases year after year, especially the ischemic CVD. So the medical public play more and more attention to ischemic CVD.At present the clinical curing fundamental of acute ischemic CVD is to clean out the thrombus, drop fibrin, resist hematoblast conglomeration , protest brain and so on. Its main purpose is to inscrease brain tissue blood perfusion.For sure the reperfusion of ischemia part is one of the keys to cure CVD, but reperfusion maybe injure the microvascular, lead to cerebral hemorrhage, hydrocephalus, aggravate brain damage. Presently the character and mechanism of the microvascular after reperfusion are still unclear. uPA is the primary enzyme species of the extrocells. When reperfusion, it can destroy the structural integrality of the microvascular. PAI is inhibitor of the uPA,whose main function is to protect the cell surface and microvascular not to be decomposed by albumen enzyme and protect interfaces between cells to keep the integrality of tissue structure. So, used the MCAO model, electropuncturing Dazhui and both Neiguan to observe the effect of uPA and PAI-1, suggesting that will supply theoretical basis for clinical practice. Method:40 SD rats were devided randomly into 4 groups as normal group, shamoperation group, model group and EA group. Block the rats' middle cerebral artery with line peg induce focal brain ischemia model. The remarks of nerve function deficit referring to Zea-Longa remarking standards. The acupoints of Dazhui and Neiguan were selected, stimulated with slow-fast wave for 30 minutes.All the rats were reperfused with 4% paraformaldehyde after being anaesthetized by 10% chloral hydrate,then taken out the brain quickly.The brain tissue 2mm away from the chiasma was cut frontally, the middle part left, immersed in hard raffin. Used immunohistochemistry SABC (spreptavidin-biotin-peroxidase complex) method to investigate the express ion of uPA and PAI-1, and the effect of...