Flavors Xiaoduyin The Pellets

Wuweixiaodu decoction, Chinese herbal, ever listed in《Golden Mirror of Medicine》includes honeysuckle flower, wild chrysanthemum, dandelion, Tokyo violet herb and semiaquilegiae radix. Modern pharmacology indicates that Wuweixiaodu decoction has curative effects on anti-inflammatory, heat-clearing and strengthening immunization. It has been generally applied to the treatment of infectious diseases which were caused by burn infection and humid heat. If Wuweixiaodu decoction is prepared into micro pellets with modern technology, its bioavailability can be improved and its curative effect must be enhanced, furthermore, the research provide ideas and methods to study micro pellets for Traditional Chinese Medicine. This research includes: (1) the establishment of analytical methods of typical principles (2) the optimization on extraction and purification process of the active constituents (3) the pharmacodynamic study of Wuweixiaodu decoction (4) the preparation method of gelatin capsule (5) the stability of Wuweixiaodu decoction gelatin capsule.According to literatures and experiments in this paper, TLC method was used to identify honeysuckle flower, wild chrysanthemum, dandelion and Tokyo violet herb. Chlorogenic acid was used as typical principles to determine the dissolution of Wuweixiaodu decoction gelatin capsule and HPLC methods were developed to determine their content in the herbals and the preparations, the dissolution exceed 90% by 45 min. All of these methods were accurate and sensitive.The extraction and purification process of the active constituents was studied by the criterion of transference rate of chlorogenic acid and the Dry-extracts content. The extracting solvents pH, extraction temperature and the soaking time were studied to investigate the influence on the typical principles' extraction. Orthogonal tests were used to optimize the decoction time, decoction times and solvent volume. Alcohol precipitation method, gel flocculating method and macro porous adsorptive resins method were used to optimize purification process and the result indicated that the macro porous adsorptive resins method was better than the others. The transference rate of chlorogenic acid was 95.51% and the Dry-extracts content was 23.65%.The optimal method of extraction and purification process had a good repeatability.The pharmacodynamics experimentation of extract of Wuweixiaodu decoction which was gotten by using the optimal extraction and purification process indicated that Wuweixiaodu decoction had definite treatment on anti-inflammatory, heat-clearing and strengthening immunization.Extrusion-spheronization technology was used to prepare Wuweixiaodu decoction pellets. Technology factors of preparing pellets and formulation factors were investigated and optimized by the influence factors test and orthogonal design test. It was discovered that the yield of the objective pellets (18-24 mesh cut) was about 83.9%.A fluid-bed spray processor was adopted for coating the pellets. The pellets were coated with Eudragit E100. The influence factors on the moisture absorption ratio were investigated. The formulation and technology was optimized.The result of stability test showed that the Wuweixiaodu decoction gelatin capsule was stable under high humidity, strong light, high temperature. Three months tests under room temperature or under accelerated circumstance indicate that the preparations were stable.