Wild Papaya Biologically Active Components Of The Hplc Study

Starntonia Chinensis had a long history of use in traditional Chinese medicine. It was used for both medicinal materials and edibles in China, and was a very important botanic resource. It had planting far and wide, year'yield was very large. Primary studies indicates that Starntonia Chinensis have many important pharmacological activities in the prevention and treatment of some cardiovascular diseases. These benefits were attributed to several active components such as flavonoids, saponins and polysaccharides etc. In the present research, a suit of analysis methods for those active components was established by using a homemade, sensitive, accurate and simple high performance liquid chromatographic (HPLC) apparatus. The HPLC system consisted of two 10ADvp high-pressure permanent flow pumps, a SPD-10Avp ultraviolet absorption detector, a RID-10Avp refractive index detector, a CTO-10ASvp column oven, a SCL-10Avp system controller and a CLASS-VP chromatogramphy workstation.The studies indicated that the effects of oleanolic acid and ursolic acid's separation and determination were optimum when determination parameters were as follows: Shim-pack VP-ODS column (250mm×4.6mm., 5μm): The mobile phase was acetonitrile-1.1%acetic acid water (94: 6); The flow rate was 0.5mL/min; UV detection wavelength was 205nm; Column temperature was the room temperature. The repressive equation of oleanolic acid was: y=3.81424×10^-8x-0.00240239, r=0.9994. The relationship of injection amounts and peak was linear in the range of 0.02-0.10mg/mL and the recovery rate was 99.83%. The repression equation of ursolic acid was: y=3.99319×10^-8x-0.000568086, r=0.9988. The relationship of injection amounts and peak was linear in the range of 0.06-0.30mg/mL and the recovery rate was 99.79%. By this method, the content of oleanolic acid from Stanuntonia Chinensis was 0.1138%, and the ursolic acid was 0.4400%.The chromatogramphic condition for the polysaccharides which in Starntonia Chinensis were: Shodex Asahipak NH2P-50 4E column (250mm×4.6mm, 5μm) ; mobile phase: acetoneitrile-water=75: 25; flow rate was 0.8mL/min; column temperature was 30°C and the detector was refractive index detector; the detect pool' temperature was 30°C. The experimental result indicated that: each monosaccharides of Starntonia Chinensis was separated good, and their molar ratio is: 0.217: 0.449: 0.334.The above methods were simple and available, accuracy and good reproducibility. The detect chromatogramphic condition was standing-by. It may be applied to the Starntonia Chinensis's active constituent content such as triterpenoid saponins and polysaccharide's analysis. Also may be use in the different habitat, the different variety and the different vegetal period of the Starntonia Chinensis's active constituent content's analysis. Moreover, it can be use to establish the corresponding quality control system about the Starntonia Chinensis product.