Chinese Medicine Of Analogues Ingredients Discrimination And The Retention Index In The Volatile Oil Qualitative Applications

Traditional Chinese medicine(TCM) has become a thriving industry,not just in China, but worldwide. In many cases, toxicity or side effectsassociated with Western Medicine have worsened human diseases, whichmake people more inclined to try natural medicine, and traditionalmethods of treatment. In the new century, TCM will meet with greatchanges in both scientific development and social life. They will createboth opportunities and challenges for TCM. Some experts have evenforecast that the 21st century will be the era of TCM, with themodernization of TCM, it may become the cornerstone of Chineseindustries. TCM is an analytical system with too much complexity. Thepharmaceutical activity of TCM come from the interactional of all thechemical components in it. The explicit characterization of chemicalsubstance has become the bottleneck in the process of TCMstandardization and internationalization.Plant essential oil is one kind of important effective components ofTCMs, whose separation and analysis is regarded to be very important. Ingas chromatography mass spectroscopy (GC-MS) analysis of complexsystems such as plant essential oils, there usually exist three problems: (1)overlapped peaks, (2) different molecules having similar or even identicalmass spectra, and (3) mass spectra of many natural compoundsunavailable in useful databases because of the difficulties of theirpurification. The present work is a systematic research, aiming at thethree problems described above.In the introduction part of this thesis, a brief review is given to therecent development of TCMs. In the view of the varieties, production,input and output marketing of TCMs, research on natural productsgradually becomes one of the hot topics all over the world now. thedevelopment of analytical science, which is closely related to the qualitycontrol and technologic standardization of TCMs, is also simplyintroduced in respect that the output of TCMs is being confronted withmany problems, especially, the realization of the standardization, modernization and internationalization of TCMs is not a trivial work inour country. The development of TCMs quality control in resent years,the application of chromatographic fingerprints, the progress of modernanalytical techniques and chemometric methods are summarized. Theintroduction of chemometrics and relative multi-resolution methodsapplied to two-dimensional hyphenated chromatographic instrumentscertainly provides an novel approach for determining chemicalcomponents from TCMs. Meanwhile, the importance and application oftemperature-programmed retention index (PTRI) in the analysis of TCMcomplex systems were expatiated simply.In chapter 2, the volatile oil in the same names of three differentplants of Muxiang was analyzed with GC/MS, and the overlapping peakswere resolved by heuristic evolving latent projections(HELP). The resultshowed that, the effective components are quite different in the eachfamily of Muxiang. One must distinguish them in using. In the thirdchapter, the two-dimensional data obtained from GC/MS is investigatedto determine qualitatively and quantitatively chemical components of theessential oil of Herb aristolochiae and Aristolochia debilis Sieb. et zucc.with the help of sub-window factor analysis (SFA). And theprogrammed-temperature retention index (PTRI) was calculated.In chapter 4, isothermal retention index(RI) and PTRI wasintroduced, the importance and application of PTRI in the analysis ofTCM complex systems were expatiated. Several examples of using I~T forisomer identification were demonstrated.