Drugs On The Volatile Oils Of Honeysuckle - Gold And Silver Vines, Dried Tangerine Peel - Citrus Aurantium And Four Sweet-scented Osmanthus

Volatile oil is one of important constituents in traditional Chinese medicine (TCM) and plants, and it has great important pharmacological effects. Volatile oil is a very complex system and contains tens and even hundreds of chemical constituents. The analysis of volatile oil is usually conducted by GC-MS, and the GC-MS data from volatile oil, however, often involves many overlapped and embedded peaks. These overlapped and embedded peaks bring many difficulties when carrying out quantitative and qualitative analysis. With the rapid development of some chemometric multi-resolution techniques such as heuristic evolving latent projections(HELP), it is possible to completely conduct the qualitative and quantitative analysis of volatile oil by the combined information on separation and identification from chromatography and spectrometry. In this study, volatile oil was the research object, and the volatile oils from herbal pair (HP) flos lonicerae-caulis lonicerae(FL-CL) and its single herbs, pericarpium citri reticulatae-fructus aurantii(PCR-FA) and its single herbs, and four osmanthus fragrans species, osmanthus fragrans var. aurantiacus (OFVA), osmanthus fragrans var. thunbergii (OFVT), osmanthus fragrans var. latifolius (OFVL) and osmanthus fragrans var. semperflorens(OFVS) were firstly separated and detected with gas chromatography-mass spectrometry(GC-MS), and then chemometric resolution methods and temperature-programmed retention indices(PTRIs) were employed to carry out the qualitative and quantitative analysis for volatile oils.44 volatile constituents of FL were identified, representing 87.22% of the total content.39 volatile constituents of CL were identified, representing 94.54% of the total content.50 volatile constituents of HP FL-CL were identified, representing 90.08% of the total content. The results showed that there were 32 common volatile constituents between HP FL-CL and single herb FL, and 33 common volatile constituents between HP FL-CL and single herb CL, and 25 common volatile constituents among FL, CL and HP FL-CL. The results also showed that 10 new constituents appeared in the volatile oil of HP FL-CL. The major volatile constituents in FL, CL and HP FL-CL were n-Hexadecanoic acid and 9,12-Octadecadienoic acid, ethyl ester, the two constituents represented 71.32%,57.33% and 84.88% of the total content of volatile oil in HP FL-CL, FL and CL, respectively.45 volatile constituents of PCR were identified, representing 99.02 % of the total content.52 volatile constituents of FA were identified, representing 98.27% of the total content.63 volatile constituents of HP PCR-FA were identified, representing 97.98% of the total content. The results showed that there were 34 common volatile constituents between HP PCR-FA and single herb PCR, and 42 common volatile constituents between HP PCR-FA and single herb FA, and 29 common volatile constituents among PCR, FA and HP PCR-FA. The results also showed that 16 new constituents appeared in the volatile oil of HP PCR-FA. The major volatile constituents in PCR, FA and HP PCR-FA were D-Limonene and 1-Methyl-4-(1-methyl ethyl)-1,4-cyclohexadiene (γ-Terpinen), the two constituents represented 80.96%,90.74% and 76.93% of the total content of volatile oil in HP PCR-FA, PCR and FA, respectively.53 volatile constituents of OFVA were identified, representing 90.87 % of the total content.58 volatile constituents of OFVT were identified, representing 89.04% of the total content.53 volatile constituents of OFVL were identified, representing 84.38% of the total content.57 volatile constituents of OFVS were identified, representing 89.73% of the total content. The results showed that there were 33 common volatile constituents among OFVA, OFVT, OFVL and OFVS. The major volatile constituents in OFVA, OFVT, OFVL and OFVS were 5-Hexyldihydro-2(3H)-furanone(γ-Decalactone), (E)-4-(2,6,6-Trimethyl-1-cyclohexen-1-yl)-3-buten-2-one((E)-β-Ionone),4-(2,6,6-Trimethyl-1-cyclohexen-1-yl)-2-butanone(Dihydro-β-ionone) and 4-(2,6,6-Trimethyl-1-cyclohexen-l-yl)-3-buten-2-ol(β-Ionol), the four constituents represented 35.84%,61.24%,29.5% and 50.53% of the total content of volatile oil in OFVA, OFVT, OFVL and OFVS, respectively.