Objectives:: Carries on HO-2 specificity Primer constructing,then observed rat penile corpus cavernosum tissue mRNA expression in the different month-old rats by RT-PCR.Method :: 40-month-old male Wistar rats were different (2,4,8,16,24) divided into five groups. Separation harvesting corpus cavernosum, including separation crus. Penis removed cartilage, the dorsal vascular tissue and the urethra to cut its longitudinal exposed corpus cavernosum. After the problem resulted from foil wrapped placed in liquid nitrogen to preserve. In liquid nitrogen ground into powder using RT-PCR method corpus cavernosum tissue 2mRNA content..Results: : HO-2mRNA expression in the corpus cavernosum increased with age (P <0.05).Conclusion: CO may play an important role in erectile function. HO-2 mRNA in the elderly might be an aspect of erectile dysfunction related mechanisms. |