Flavored Pulsatilla Decoction On Experimental Study Of The Treatment Effect And Its Mechanism In Rats With Ulcerative Colitis

Objective:Ulcerative colitis(UC)is an idiopathic chronic inflammatory bowel disease(IBD).Its main manifestations are diarrhea,bloody mucopurulent stool,abdominal pain and tenesmus,which is lingering difficult to cure and repeated attack.UC is regarded as in close relation with the occurrence of colon carcinoma.In recent years,the immunological mechanism of the pathogenesis of UC in modem medicine has attracted a wide spread attention and made much progress.But because the pathogenesis of UC is not completely clear,there is not one kind of treatment medicine of remarkable curative effect and small side effect at present.Modified BaiTouWeng decoction is professor Qiu Mingyi's experienced prescription based on BaiTouWeng decoction.According to many years' clinical experiences,it has good curative effect on UC.It has been proved by the previous experiments of our team that BaiTouWeng decoction has good curative effect on UC model rats.Thus,we plan to study the therapeutic effect and mechanism of modified BaiTouWeng decoction treating UC by setting up UC rat model,especially we observe modified BaiTouWeng decoction to affect Expression of cytokine and adhesion molecule on basis of previous research,which reveals deeply the action mechanism of modified BaiTouWeng decoction treating UC.That can provide some experimental basement and theoretical evidence for traditional Chinese medicine (TCM)treating UC,and then lay a foundation for revealing the basic rule of TCM treating UC to make the breakthrough,which can provide the theory instruction for the clinical medication.Methods:In the experiment,eighty four SD class healthy rats were randomly divided into six groups.Every group had fourteen rats(seven rats were male,seven rats were female),which there was no significant difference in the rats' weight between each group(P＞0.05).The first group,the second one,the third one, the fourth one and the fifth one was the high dose group of modified BaiTouWeng decoction,the medium dose group of modified BaiTouWeng decoction,the low dose group of modified BaiTouWeng decoction,the SASP group and the model group respectively,and the sixth group is the normal group.Except the normal group,the other five groups were made through the integration of general immunity and local stimulation(DNCB and acetic acid)to create the rat model of UC.After the model was successful,the six groups were used the high,medium and low dose of modified BaiTouWeng decoction,SASP and distilled water respectively to treat by gastric perfusion in 15 days.Then we observed the changes in general status and pathological changes in colon tissue of UC model rats.The expression of local immunological index such as IL-8,IL-13,CD44,CD54 in lesions of colon mucosa were detected with immunohistochemical method.Results:(1)The rats in the model group can been observed the changes such as mucus dilute stool and bloody purulent stool,emaciation,laziness,intolerance of cold,no luster in hair,poor appetite,loss of weight and increase of colonic damage scores.Among them,compared with the normal group,there was more significant difference in the weight and colonic damage scores in the model group(P＜0.01).After treatment,there was some improvement in different degree in symptoms in every treatment group.Compared with the normal group,there was more significant difference in the weight and colonic damage scores in every treatment group(P＜0.01).Among the treatment groups,the best effect was the high dose group of modified BaiYouWeng decoction(compared with the SASP group in colonic damage scores,P＜0.01);the effect of the medium dose group of modified BaiTouWeng decoction was equivalent to that of the SASP group(compared with the SASP group in colonic damage scores,P＞0.05).The pathological section showed that:there was more difference in local loss of colonic mucous membrane,gland arrangement being regular or not,mucosa hyperemia and inflammatory cell infiltration and so on between the model group and the normal control group.Meanwhile,compared with the model group,there was also more difference in those in the three modified BaiTouWeng decoction groups and the SASP group.(2)The level of IL-8 in rats' colon tissue in the model group was significantly higher than that in normal group(P＜0.01);the level of IL-13 in rats' colon tissue in the model group was significantly lower than that in normal group(P＜0.01).After treatment,the level of IL-8 in rats' colon tissue in every treatment group was significantly lower than that in the model group(P＜0.01);the level of IL-13 in rats' colon tissue in every treatment group was significantly higher than that in model group(P＜0.01).Among the treatment groups,the best effect was the high dose group of modified BaiTouWeng decoction(compared with the SASP group in the level of IL-8 and IL-13,P＜0.01);the effect of the medium dose group of modified BaiTouWeng decoction was equivalent to that of the SASP group(compared with the SASP group in the level of IL-8 and IL-13,P＞0.05).(3)The level of CD44 and CD54 in rats' colon tissue in the model group was significantly higher than that in normal group(P＜0.01).After treatment,the level of CD44 and CD54 in rats' colon tissue in every treatment group was significantly lower than that in the model group(P＜0.01).Among the treatment groups,the best effect was the high dose group of modified BaiTouWeng decoction(compared with the SASP group in the level of CD44 and CD54,P＜0.01);the effect of the medium dose group of modified BaiTouWeng decoction was equivalent to that of the SASP group (compared with the SASP group in the level of CD44 and CD54,P＞0.05).Conclusion:The result of the experiments above shows that(1)This model made with DNCB and acetic acid is successful.(2)It is obvious that IL-8 and IL-13 are important role on UC,which the level of IL-8 increases and that of IL-13 decreases in colonic mucosa of UC.Modified BaiTouWeng decoction can regulate the abnormal immune response by decreasing the level of IL-8 and increasing the level of IL-13 in colonic mucosa of UC.(3)It is obvious that CD44 and CD54 are important role on UC,which the level of CD44 and CD54 increases in colonic mucosa of UC. Modified BaiTouWeng decoction can regulate the abnormal immune response by decreasing the level of CD44 and CD54 in colonic mucosa of UC.(4)Modified BaiTouWeng decoction has the better curative effect on UC.