Shennong Xiangju Chemical Composition And Fingerprint Study

Dendranthema indicum var aromaticum is the dry capitulum of compositae plant Shen Nong fragrant chrysanthemum,which produces to the Hubei Shennongjia area,distributes toward the sun open in elevation 2,000 meter above spacious area,it was one kind of new resources plant, was a new variety,in Dendranthema compositae.It entirely had the special full-bodied fragrance,the folk maked its flower and leaf which dried in the shade as the sachet,heat-clearing drugs and the treatment in chronic hepatitis.Bendranthema indicum var aromaticum is the perennial herb.The plant is diminutive,entirely covered with the white fluff,the flower,leaf or radical sparsely had the full-bodied fragrance.Stem's base belt is purple red;The leaf blade is much small and thick,the bottle-green,above the leaf,vein sticks up,under it small gland is hollow;The flower is small, yellow.This kind is approximate to the chrysant-hemumindicum (D.indicum),the folk called it as the small mother chrysanthemum, comparing with the original variety,the main difference is:The Dendranthema indicum var aromaticum's leaf,the flower and so on have the full-bodied special fragrance.The chemical analysis and the pharmacology experiment preliminaryresult indicated that:Dendranthema indicum var aromaticum's flower,the leaf include alpha-thujone,beta -thujone and the borneo camphor and so on, a kind of terpene compound,but widely uses in industry,chemical industry, drink and medicine,has the certain development and useful value.Its flower,leaf can alleviates fever the disintoxicating effect,the folk uses it in treating the cold,the pharynx and larynx which is swollen and painful,the eye which hot and so on,is onekind of the development medicinal plant new resources which is worth studying.It used to prevent and control the cold,the headache,bac-illary-dysentery,and coronary disease and hypertension,the Dendrant-hema indicum var aromaticum also has the function on sterilization,treating in disorders of the liver, dispersing the wind,lowering the blood pressure in the West Hubei mountainous area.At present,although Dendranthema indicum var aromaticum shape in groups in the Shennongjia distribution area,has the quantity,but thedistribution scope is not big,and the resources is limited,we shouldstrengthen the protection,positively carrie on the artificial propagation and the cultivation,reasonably develops the use.It can be used in treating the chronic hepatitis in the future.The thesis study on the Dendranthema indicum var aromaticum's pharmacognosy aspect research,has made a more detailed description on its raw material for medicine character and the microcharacteristic, provided some beneficial bases for further studying on the research and the application of Dendranthema indicum var aromaticum.At the same time,we has carried on the separation and the purification to the Dendranthema indicum var aromaticum in the chemical composition by using the silica gel column chromatograph;According to the physics and chemistry nature and the spectrum data(IR,MS,UV,¹H-NMR,¹³C-NMR), we carries on the structure appraisal;has separated and appraised 5 compounds from the Dendranthema indicum var aromaticum,respectively was:acacetin,apigenin acacetin-7-O-β-D-glucopyranoside,apigenin-7 -O-β-D-glucopyranoside,luteolin,β-sitosterol,daucosterol,and acacetin,acacetin-7-O-β-D-glucopyranoside and apigenin-7-O-β-D-glu copyranoside were reported firstly in this plant.Determine the content of chlorogenic acid and luteolin in Dendranthema indicum(L.),study out limits of chlorogenic acid and luteolin in Dendranthema indicum(L.),The method is simple and accurate with a good reproducibility,and can be used as quantitative analysis method for this preparation.Finger print of Dendranthema indicum var aromaticum was established by HPLC,with system adaptability and methodology consideration,which has the advantage of systematism,allsidedness and characteristic.The method can be used to Dendranthema indicum var aromaticum efficiently and evaluate the quality of all sources of them.This method can be used to evaluate and control the quality of medicine wholly.