Investigation Of Vascular Endothelial Growth Factor(VEGF)Concentration In Plasma,Aqueous Humor And Vitreous With Diabetic Retinopathy

Diabetes Mellitus (DM) is characterized by glucose metabolism disorders and may affect almost all the organs and tissues of the body. Diabetic retinapathy (DR) is a major ocular complication of diabetes mellitus. The most important causes of blindness are repeated vitreous hemorrhage induced by neovascularization, traction retinal detachment and other complications. Now the research of DR has been developed from clinical manifestation and pathogenesis to molecular biological fields. The results of these study identify that several growth factors, especially vascular endothelial growth factor (VEGF), play roles in neovascularization. Generally VEGF may promote endothelial cell proliferation, increase blood vascular permeability and induce neovascularization. And VEGF stimulates an excessive neovascularization under abnormal circumstances. Lots of experiments suggested that VEGF in vitreous and retina of patients with proliferative diabetic retinopathy (PDR)~[1-4] played a great role in the development of PDR. But up to now we don't know so much about the VEGF concentration in plasma, aqueous humor of patients with DR. This study is trying to find if the changes of VEGF in plasma is associated with the development of PDR, and further to find the relations of VEGF concentration in plasma, aqueous humor and vitreous in patients with PDR. [Objectivej To investigate the relations between the concentration of VEGF in plasma and the development of DR; to determine the relations between the changes of VEGF concentration in plasma, aqueous humor and vitreous of patients with DR and PDR,as well as to find if the condition of metabolism control of patients with DR mayaffect the VEGF level.lMethod]Double antibody sandwich enzyme-linked immunosorbent assay was used tomeasure the levels of VEGF concentration in plasma of non-diabetic retinopathy(NDR) patients group, background diabetic retinopathy (BDR) patients grouP, andproliferative diabetic retinopathy (PDR) patients grouP. The level of HbAlc in plasmaof patients in above thIee grouPs were measured at the same time. The VEGFconcentration in aqueous humof, vitreous of PDR patients group and normal groupwas also measured fOr driher comprehensive analysis. Human VEGF inununoassayQuantikine with high sensibility and quality was imported from R & D Co. LTD.,USA.lResultll. VEGF concentration in plasma of DM patients grouP was obviously lower than thatof normaI group (p<0.0l ).(l)There was no significan difference in VEGF concentration of plasma betWeenNDR and DR patients groups.(2) There was no significant difference in VEGF concentration of plasma betweenBDR and PDR patients groups.2. There was an increasing tendency in the VEGF concentration in aqueous humor ofPDR patients group. There was no significan statistic difference as it wascompared with normal grouP (P>0.05).3. The VEGF concentration in vitreous of PDR patients group was obviouslyincreased, there was significam difference as it was compared with normal grouP(P<0.0l).4. It showed that there was significant difference between VEGF concentration inplasma and that in vitreous of the 6 PDR patients selves (p<0.05). Bllt there was nocorrelations between the VEGF concefltration in plasma and vitreous (rr0.l19,P>0.05).5. It showed an increasing tendency of VEGF conceniYation in tal in plasma,aqueous humor and vitreous of the 3 PDR patients selves, but there was nosignificant difference to>0.05).6. It showed that there was significant difference among VEGF concentration inplasma, aqueous humor and vitreous of all the PDR patients (p<0.01). In normalgroup there was no significan difference axnong VEGF concelltration in plasma,aqueous humor and vitreo...