Parameters Of Intraoperative Capsule Treatment Spleen Metastasis Of Gastric Cancer Mouse Model, Gene Expression Profiling Studies

Objective:To study differential expression profile induced by the model with spleen-deficiency and gastric cancerometastasis in mice and to explore its molecule-biological character;to study differential expression profile induced by treatment of Shenzhu Capsule on the model with spleen-deficiency and gastric cancerometastasis in mice and to analyze its molecule mechanism of therapeutics on spleen-deficiency gastric cancer.Methods:On the basis of establishment of spleen-qi deficiency models by feeding BALB/c-nu/nu mice with vinegar,an animal model with combination of disease and syndrome called model mice with spleen-deficiency and gastric cancerometastasis was established by adopting SOI to transplant SGC9701 gastric cancer tissure after generation cultivation on the tunica serosa gastria of the mice. Gene chips were used to detect differential expression of spleen-deficiency gastric cancerometastasis mice and normal mice,Weifuchun treated mice and model mice as well as Shenzhu capsule treated mice and model mice.Results:The model with spleen-deficiency and gastric cancerometastasis in mice could upregnlate 151 genes and downregnlate 53 genes,which were involved in pathways such as MAPK signaling pathway;Shenzhu capsule,whose pharmacodynamic action was involved in pathways such as VEGF signaling pathway could retroregulate differential expression of 9 genes caused by spleen deficiency gastric cancer.Conclusions:The model of spleen-deficiency and gastric cancerometastasis in mice was mocular-biologically characterized as a model of both of gastric cancer and spleen-deficiency.Shenzhu capsule treated spleen deficiency gastric cancer and improved the body by regulating expression of metabolism related genes,ion channel and transport protein,el ta.and from pathways such as VEGF signaling pathway and MAPK signaling pathway.