Aconite Compatibility Rhubarb Attenuated Efficiency Material Basis For A Preliminary Study

Objectives:To study the content changes of each index components after compatibizing Radix Aconite Lateralis Preparata and Rhubarb.At the same time,to study the material base of detoxication and synergism after the compatibility of Radix Aconite Lateralis Preparata and Rhubarb.Methods:In the direction of clinical application of Radix Aconite Lateralis Preparata, selecting the content and dissolution rate of "Total Alkaloids- Ester Alkaloids-Diester Diterpenoid Alkaloidas(including Mesaconitine,Aconitine and Hypaconitine)" as indexes,we study the content changes of each index components and systematically discuss the influences which different factors affect on the three Alkaloidas.In the process,we choose main processing drugs(including Blcak PRA,White PRA,Crude PRA ) as objects and apply analtical technique of UV,HPLC,HPCE and LC-MS to handle data.All analyses are proceeded in three layers as follow:compatibility of medicinal substances,compatibility of active ingredient and compatibility of single ingredient.Results:1.The decocting time has large influence on content and dissolution rate of these three Alkaloidas.The complex index is stable after 6 hours when decocting Radix Aconite Lateralis Preparata separately and the same index is stable after 2～4 hours in case of compatibility of Radix Aconite Lateralis Preparata and Rhubarb(1:1). The result can be suggested as the best clinical decocting time because this result coincides with that of acute toxicity experiment.2.The change of alkaloids in water decocting solution is obvious and it reflects the increase of the total alkaloids dissolution as the decrease of the Ester Alkaloids dessolution in case of the compatibility of Radix Aconite Lateralis Preparata and Rhubarb.The compatibility of total alkaloids' active ingredients and Rhubarb ingredients indicates the anthraquinone,tannin,polysaccharide and salt have influence on the hydrolysis of the Ester Alkaloids.The compatibility of single ingredient indicates the saccharide,salt,trioxymethylanthraquinone,tannic acid, different solvent and different pH have influences on the hydrolysis of the Ester Alkaloids.The affecting trend is same as that of compatibility of medicinal substances and compositions.3.The different compatibility ration of Radix Aconite Lateralis Preparata and Rhubarb has influences on the hydrolysis of Diester Diterpenoid Alkaloidas.And the effect result indicates some regularity.4.The increase of polysaccharide dessolution partially shows the promotion of acid solution in case of the compatibility of Radix Aconite Lateralis Preparata and Rhubarb. 5.We have set the technology of extracting and purifying Radix Aconite Lateralis Preparata polysaccharides and determine the composition of monosaccharides in Radix Aconite Lateralis Preparata polysaccharides.We have concluded the result as follow:the Radix Aconite Lateralis Preparata polysaccharides is composed of Glu and Gal with the composation ration is 16.55:28.72.Conclusion:We have suggested the best clinical decocting time of decocting Radix Aconite Lateralis Preparata separately and compatibility of Radix Aconite Lateralis Preparata and Rhubarb(1:1).When decocting Radix Aconite Lateralis Preparata,the dessolution and hydrolysis of toxic components is a complicated organic process.This process is affected by synthetic action of saccharide,salt,solvent and pH and it is an important factor which affects the toxity of Radix Aconite Lateralis Preparata.The experiment shows the compatibility of Radix Aconite Lateralis Preparata and Rhubarb can promote the dessolution of total alkoids and the hydrolysis of Ester Alkaloids.So the compatibility also can enhance the pharmacodynamic action and neutralize poison.