Hepatoprotective Activity Components Of The Grass Coral Research

Sarcandra glabra(Thunb.) Nakai[syn.Chloranthus glabra(Thunb.) Makino] belongs to the genus Sarcandra of Chloranthaceae,which is distributed in Anhui, Zhejiang,Jiangxi,Fujian,Taiwan,Guangdong,Guangxi,Hunan,Sichuan,Guizhou and Yunnan Provinces.The whole plant was used as a drug for anticancer,antibacterial, antivirus and increasing the level of immunity.We separated the 70%alcohol extract of Sarcandra glabra on macroporous resin D101 using water and 30%,70%,95% EtOH-H₂O to elute.The result of bioassay displayed that the 70%EtOH- H₂O elution showed potent hepatoprotective activity.So we separated the 70%EtOH- H₂O elution on a polyamide column eluted with water and 30%,60%EtOH-H₂O,acetone to give four fractions.The subsequent bioassay showed that the polyamide 30%,60%EtOH-H₂O fractions have remarkable potent hepatoprotective activity.In order to find the compounds have hepatoprotective activity from Sarcandra glabra,we studied the polyamide 30%,60%EtOH-H₂O fractions systemically in chemistry by means of chromatography methods and spectral technologies.Sixteen compounds were obtained and identified as 7 sesquiterpenoids(1-7),2 coumarins(8-9), 1 flavonoids(10),3 phenolic acids(11-13),3 lignans(14-16).Their structures were determined as:8β,9α-dihydroxyeudesman-4(15),7(11)-dien-8α,12-olide(1), 8β,9α-dihydroxylindan-4(5),7(11)-dien-Sα,12-olide(2),8α,9α-dihydroxylindan-4(15), 7(11)-dien-8α,12-olide(3),chloranoside B(4),chloranthalactone E(5),(-)-istanbulin A (6),atractylenolideⅢ(7),isofraxidin(8),hemidesmin-1(9),(2R,3R) -3,3',5,5',7-pentahydroxyflavanonol(10),methyl rosmarinate(11),ethyl rosmarinate(12), N-trans-feruloyltyramine(13),(7S,8R)-dihydrodehydrodiconiferyl alcohol(14), (7S,8R)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside(15), glochidioboside(16).Among them,compounds 1-3 are new sesquiterpenoids, compounds 9-10,12-14 were isolated from this genus for the first time.Compounds 3 and 7 showed hepatoprotective activity against D-GaiN-induced toxicity in WB-F344 cells.Compounds glabraoside A and glabraoside B are phenylpropanoid-substituted catechin glycosides isolated from the D101 30%EtOH-H₂O elution,which showed potent hepatoprotective activity in our pharmacological experiment in vitro.We found that the D101 70%EtOH-H₂O elution also had this kind of constituents by HPLC analysis.It is hard to get a large quantity of them by means of chromatography methods because their structures contain many phenolic hydroxyl groups.So it is necessary to synthesize or semisynthesize them to supply enough quantity for pharmacological research in vivo.This paper discussed the synthetic method of compounds glabraoside A, glabraoside B and their glucoside derivants.