Versicolor Fruiting Bodies Of Polysaccharide And Mycelium Polysaccharide Separation And Purification

Coriolus versicolor(L.ex Fr.) Quel is a traditional Chinese medicine and edible fungus which is a Polyporaceae fungus belonging to the Basidiomycetes. The modern Phytochemistry and Pharmacology research show that it contains many chemical compositions,such as protein,fat,polysaccharides(CVP), Polysaccharidiopeptide(PSP),dextran,lignin,amino acids and mineral salt,ect. The CVP or PSP are its active components.CVP or PSK with a wide range of physical activity,can inhibit the tumor and improve immune function,protect liver, anti-ulcer,and cause no toxic or side effects to normal cells.It is also a new anti-tumor immunoregulant.This research mainly focused on the molecular,component and structure characterization of Coriolus Versicolor Polysaccharide(CVP) and Coriolus versicolor mycelium polysaccharide(PSP) by means of many kinds of modern chromatograph and spectrum technologies.Four water-soluble homogeneous polysaccharides,named CVP-a,CVP-b,CVP-c and PSP-a were purified from Coriolus Versicolor Polysaccharide(CVP) and Coriolus versicolor mycelium polysaccharide(PSP) through grading precipitation with ethanol,DE-52 cellulose chromatography and gel-filtration chromatofraphy repeatedly.HPGPC was used to analyze their purity and determine their molecular weight(Mw).Thin layer chromatography(TLC),GC-MS,infrared spectrum(IR),and NMR were applied to analyze the configuration of glucose..It was showed by the HPGPC that each peak of the four fractions was a smooth balanced single one.So it was deduced that CVP-a,with Mw is about 1.5×10~4,CVP-b,with Mw is about 1.2×10~4 and CVP-c, with Mw is about 1.1×10~4,were all composed of glucose,meanwhile the configuration of glucose wasβ.And PSP-a,with Mw is about 1.7×10~4,was also a composition of glucose,but the configuration of glucose wasβ.This paper compared the Coriolus Versicolor Polysaccharide(CVP) and Coriolus versicolor mycelium polysaccharide(PSP) systematically.Methods of detecting the average molecular weight were established by means of HPGPC using the index of retention time.Precipitated with 60%ethanol,dealed with DE-52 cellulose chromatography and analyzed by HPGPC,CVP and PSP coming form different manufactories and variaties could be distinguished..The method brought up ideas and ways for quality control of Fungi polysaccharide drugs.