Adenine And Epinephrine Interaction

DNA is the basic genetic material in biology,the carrier of genetic information and the material base of gene expression,which plays decisive function to the biological evolution and decides the direction of biological development.Adenine,one of the two important purines found in nucleic acid,which is heterocyclic compound including the nitrogen atom and is in the conjugate-system,is vital to the biological system.Adrenaline is an important neurotransmitter of mammals and human beings,which controls both the nervous system to carry out a series of biological reactions and nerve chemical processes.Adrenaline is also a kind of biological molecules.The research of intermolecular mutual function of biology is the foundation of understanding the biological system's special function and distinguishing the mechanism.To study the interaction between alkali base and adrenaline can propel the development of biology, pharmaceutical chemistry and physical chemistry,etc.In this paper we used electrical chemistry method to study the cyclic voltammetry of adrenaline with adenine,meanwhile the quantum chemistry method was used to investigate the interaction of hydrogen bonds between adrenaline and adenine.Cyclic voltammetry was used to study the effect of adenine on the cyclic voltammetric properties of adrenaline in the hydrochloric acid system,the sulfuric acid solution,the phosphoric acid buffer solution,the B.R.buffer solution as well as in the KRPB buffer solution. The results indicated that the adenine had different stabilization in different systems to adrenaline.Along with adenine increased,the oxidization peak potential was moving,the deoxidization peak potential was negative moving and the peak electric current was reducing, namely the ability of oxidation of adrenaline was decreasing.And ionic strength also has certain effect on the reversibility of the electro-oxidation behavior of adrenaline.Simultaneously we had studied the adenine around the influence on the adrenaline oxidation behavior using the cyclic votammetry and the alternating-current impedance in hydrochloric acid solution with constant ionic strength and different pH value.The results indicated that the pH value of solution had certain influence to the interaction between adenine and adrenaline.The interaction between them increased along with the pH value of solution enlarged.Density functional theory method was used to study the hydrogen bonding complexes formed between adenine and the protonizing adrenaline.The structure including amino is the main existence of adrenaline,we optimized its configuration with quantum chemistry method. Meanwhile,seventeen of the hydrogen bonding of complexes formed between adrenaline and adenine have been optimized at B3LYP/6-31+G(d) basis set level.Parameters such as binding energies,bond lengths and bond angles were also obtained.The calculation results showed that the formation of hydrogen bonds between adenine and adrenaline had not only reduced system's energy,but also had increased the stability of adrenaline and the ability of oxidation of adrenaline decreased to a certain extent.The calculation results could be better used to explain the experimental phenomena that adenine had certain influence on the oxidation behavior of adrenaline.