Born Drink On Renovascular Hypertension In Rats Of Tgf-beta <sub> 1 </ Sub> And Il-1¦Â Levels Of The Experimental Study

Objective:To observe the effects of Tianshengyin on the expressions of TGF-β₁ and IL-1βin the renovascular hypertensive rats.Method:Sprague-Dawley rats with clean class were randomly subdivided into normal,sham-operated and operated group.After one week adaptability feeding,the rats were copied with two-kidney one clip(2K1C).In the operated group for renovascular hypertension,the rats underwent the operation via nipping the left renal artery.In the sham-operated group,the rats underwent the operation with only separating the left renal artery.Meanwhile there were no management on the rats in the normal group.Four weeks later,The rats in Tianshengyin group were fed with Tianshengyin,While the ones in model group, sham-operated and normal group were fed with equal volume of distilled water respectively.The blood pressure recorded at 9 time points respectively,i.e.the time points of pre-operation and post-operation from the first week to eighth week.And observe the levels of TGF-β₁ and IL-1βin the time points of pre-operation and post-operation(the forth week,sixth week,eighth week).All rats were sacrificed in the eighth weekend.The left kidneys were collected,and stained by Hematoxylin-Eosin for morphological evaluation。 Results:Compared with the sham-operated group and the normal group,the blood pressure,and kidney fibrosis in model group elevated. There was markedly statistical difference between these groups(P＜0.01). After treatment with Tianshengyin for four weeks,we found that compared with model group,The blood pressure had lowered 12.2mmHg, but there was no markedly statistical significance(p＞0.05).The degree of kidney fibrosis ameliorated in treated group,the levels of TGF-β₁ and IL-1βdecreased significantly(p＜0.05).Conclusion:These results suggest that Tianshengyin could inhibit renal fibrosis probably by means of decreasing the expression of TGF-β₁ and IL-1βin the kidneys.