Acupuncture Reinforcing And Reducing Method Twisting The Rbc Rat Model Of Peripheral Blood Deficiency Syndrome, The Epo Impact

When treat the disease with acupuncture, except choose the appropriate points in accordance with syndrome differentiation, acupuncture manipulation is the key to improve the needle feeling and the curative effect. After acuesthesia, the method of inspiring body's vital qi, letting the lower function recovery to normal called reinforcing method; the method of discharging pathogenic evils and letting the higher function back to normal called reducing method. The role of technique of acupuncture manipulation in ancient traditional dissertation or in the modern scientific research were all had been sure, Acupuncture manipulation is also the important part of using the acupuncture in therapy obey the principle of reinforcing deficiency and reducing excess. Twirling reinforcing and reducing manipulations had been recorded large difference by ancient physicians, in later generations the manipulations had been learned difference. Therefore, using modern experimental research on twirling reinforcing and reducing manipulations effectively demonstrates technique is necessary. In the treatment of blood deficiency syndrome in TCM, always mainly invigorating qi and blood, balance the yin and yang, culture the spleen and stomach for enriching blood and regulating zang-fu functions of body, so choose the points on spleen and stomach meridian line for reinforcing method.Objective:Using different technique and other stimulation method for blood deficiency syndrome rats observe the change of red blood cells (RBC) quantity, and serum erythropoietin (EPO). Explore the effectiveness of twirling reinforcing and reducing manipulations, and the difference of reinforce method and the reducing method, preliminary analysis and research for the mainly mechanism of therapy the blood deficiency syndrome in acupuncture.Methods:This experiment with twirling reinforcing and reducing manipulations effect on blood deficiency syndrome rats, Randomly divided male SD rats into blank group, model control group, acupuncture group, reinforcing group, reducing group, and moxibustion group, the acupuncture group, reinforcing group, reducing group, and moxibustion group divided into 5d,10d,15d,20d four subgroup, the model control group divided into 5 subgroup, except 5d,10d,15d,20d, it had Od for more. Choose Housanli point. To each time point, weight the rats, record the time of endurance swimming in pool. After anesthesia, taken blood from the abdominal aorta, count the RBC quantity, and measure the EPO content in serum by ELISA. Use SAS statistical software for results.Results:Compare the number of RBC, model control group and the other groups were rose, until 20 day there were no difference in each group. The reinforcing group grows up most quickly. In 5d, it's has been more than other groups (p<0.05), the acupuncture group and reducing group were followed, the moxibustion group has been the last. In 15d there were obvious difference between the blank group and the model control group (p<0.05). The reinforcing group had recovered fastest in treatment groups. Compare the content of EPO, the all decline after molding, and then increase. The model control group is lower than other treatment groups (p<0.05). In 20d, the other groups were lower than blank group (p<0.05). In each treatment group, the reinforcing group EPO has increased more than others, it was the best of all (p<0.05). Acupuncture group better than reducing group, moxibustion group grow fast in 10d. Compare the time of endurance swimming,5d the model control group was shortest (p<0.05), the acupuncture group and reinforcing group longer than reducing group and moxibustion group (p<0.05), the reinforcing group better than reducing group (p<0.05). 10d the blank group is the best, the model control group was shorter than reinforcing group and moxibustion group (p<0.05), the reinforcing group better than reducing group (p<0.05).15d there were no difference in each group.20d the reinforcing group was the best, the reducing group was the shortest, the time of reinforcing group and acupuncture group even longer than blank group (p<0.05).Conclusion:(1) After acupuncture and moxibustion therapy, the treatment groups had increased obviously than model control group in peripheral RBC quantity. (2) After acupuncture and moxibustion therapy, the treatment groups had increased obviously than model control group in the content of serum EPO, acupuncture and moxibustion treatment promoted the rapid increase of EPO secretion. (3) After acupuncture and moxibustion therapy, the treatment groups had obviously faster than model control group in endurance swimming time, and the strength and endurance recover faster, (4) The blood deficiency syndrome model rats'blood deficiency syndrome had been improved, the strength and endurance had been recovered, hormone levels had been enhanced after acupuncture and moxibustion therapy. This was a preliminary discussion of mechanism for acupuncture and moxibustion treatment for blood deficiency syndrome. (5) The manipulations of twirling reinforcing and reducing had difference effects, compare of the effect of acupuncture therapy blood deficiency syndrome between reinforcing group and reducing group, the reinforcing group is better. (6) The method of the thumb force to move forcefully twisting 360°, then automatically return after acuesthesia, frequency 20 cycles/minute; the thumb force back force twist 360°, and then automatically back forward, frequency 20 cycles/minute, this effective method can be seen as one discuss of manipulations of twirling reinforcing and reducing.