| ObjectiveStudy the effect of Chondroitin sulfate on brain damage of rats in chronic alcoholism.Methods1. Animals model establish:Sixty male Wistar rats were assigned to six groups: blank group was given with distilled water, alcohol model group was given 50 % alcohol of 8 ml·kg-1·d-1 by intergalactic infusion, naloxone group was given naloxone 0.08mg·kg-1·d-1by intraperitoneal injection after given alcohol thirty minutes later, Chondroitin sulfate low, medium and high dose group was supplemented with Chondroitin sulfate 50,100 and 150 mg·kg-1·d-1 respectively besides treaded as the alcohol model group. All rats were treated 2 weeks; after 2 weeks the dose of alcohol was increased to 12 mg·kg-1d-1. Eight weeks later, animals were forbidden feeding 12 hours and given water freely, after the anesthetic, take blood serum and brain tissues were removed on glacial table. All samples were stored at -80℃until analyzed.2. Observation of brain tissues morphology:Rats brain tissue with formaldehyde 24 hours, embedded in paraffin, making pathological section, HE stain. Rats nerve cells was observed with light microscope.3. Determination of biological index:Glutathione peroxidase (GSH-PX), activity of superoxide dismutase (SOD), Malondialdhyde (MDA) and activity of acetyl cholinesterase (Ache) in brain tissues were measured by biochemical method with kits.4. Determination the content of NE,5-HT and DA:by High Performance Liquid Chromatography.Results1. It was observed that nerve cells decreased obviously and nerve cells arranged disorder in cerebral cortex and hippocampus in model group. Nerve cells arranged was clear in cerebral cortex and hippocampus in CS middle dose group. 2. The activity of SOD and GSH-PX in serum and brain tissues decreased obviously in model group, the activity of SOD and GSH-PX in serum and brain tissues in CS middle group and high dose group increased obviously; the content of MDA in serum and brain tissues increased in model group, the content ofβ-EP increased in model group; the content of MDA in serum and brain tissues decreased in CS middle and high dose group; the activity of Ache and the content ofβ-EP decreased in brain tissues in CS middle dose group and CS high dose group;3. The content of NE,5-HT and DA in brain tissues in CS low dose group decreased; the content of NE,5-HT and DA in brain tissues in CS medium group and CS high dose group decreased obviously.Conclusions1. The activity of SOD and GSH-PX increased in serum and brain tissues in CS medium group and CS high dose group, the content of MDA in serum and brain tissues in CS medium group and CS high dose group decreased. CS may have anti-oxidant free radical scavenging capacity, the impact of chronic alcoholism in rats'in vivo lipid peroxidation, to a certain extent, prevented lipid peroxidation and prevent further damage free radicals on the cells, and thus play a role in chronic alcoholism.2. The transmitter content of NE, DA,5-HT and activity of Ache in brain tissues in chronic alcoholism rats decreased in CS medium group and CS high dose group, the content ofβ-EP in brain cortex decreased, By regulating the levels of neurotransmitters and reduce the contents ofβ-EP in chronic alcoholism rats, regulating abnormal nervous system, reducing brain damage. |
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