Purpura Particle Intervention Experimental Itp Mouse Model Of Experimental Research

Purpose:Explore purpura particles itp model intervention of the mouse,discussed the possible role of the mechanism,for the rational application for an experimental basis.Material and method:SPF level BALB/c mouse (male and female) were modeling 1d, 3d, 5d, 7d, 9d, 11d, 13d intraperitoneally APS200ul, the establishment of chronic ITP mouse model. Measured before model BALB/C mice peripheral platelet counts, according to the peripheral platelet count will BALB/C mouse were randomly divided into 5 groups:nor- mal group, model group, control group, comparison group,purpura particle group . 12 in each group, male and female half and half. Normal group, model group, while modeling fed with dis- tilled water, the other groups at the same time modeling for the corresponding drug. 1 ti- me per day for consecutive 15 days.Administration also observed activities and eating mice, body weight and food intake time weighing.Modeling methods used by the immune injection of exogenous anti-platelet an- tibodies in mice to establish animal models of ITP. After the last administration 24h,eyeball blood,and anticoagulant mixing,automatic analysis of five multi- species detected in peripheral blood platelet count. Mice were killed by cervical dislocation, open the chest and abdomen,follow- ed by clipping the thymus, spleen and weighing, according to the formula were the thymus index and spleen index.Enzymes immune (ELISA) method of detecting TPO, TGF-β1 a content.Results:1. ITP mouse on the general condition and weight, the impact of diet.Before modeling of the mouse lively, responsive, active water and food, fur luster; body wei- ght and food intake was not significant (P>0.05). 2 days after modeling, subcutaneous purp- ura, accompanied by the spirit of malaise,unresp- onsive, slow reaction to external stimuli,re- duced activity, emaciation, hair loose, eyes, ears, tail is pale, get together and tired of lying, eating into the water reduce symptoms such as loose stools thin. Treatment for 15 days.2. ITP mice on peripheral platelet count and parameters. Compared with model group, the treatment group were peripheral platelet counts were signif- icantly increased (P<0.05, P<0.01); were significantly reduced mean platelet volume (P<0.05); purpura ITP particle model can be increased significantly smaller mouse peripherral platelet counts, reduced mean platelet volume, improve platelet function.3. On the ITP mouse thymus index, spleen index of.Spleen index decreased; thymus Indices were statistically significant (P<0.05) significant difference.4. Right mouse ITP model out of the week in the blood TPO,TGF-β1 a content of Model set the tpo mouse week in spite of the rising trend was not significant (P>0.05). Purpura particles can reduce the blood of the week, a content TGF-β1 were statistically significant (P<0.05).Conclusion:Study on scattering neutron background under specific condition of experiment:1 .Purpura particles have significantly itp platelet the week,reduce the volume of platelet function ;2 .Purpura particles have reduced spleen index, upper the role of the thymus index ;3 .Purpura particles by adjusting tgf, beta, to a content itp effect.