Found The Wind Expectorant Medicine Ang Ii-induced Expression Of Human Umbilical Vein Endothelial Cells, The Lox-1 Mrna And Inosmrna

Purpose: To investigate the AngII on human umbilical vein endothelial cells Lox-1 mRNA and iNOSmRNA expression, to clarify the law found the wind expectorant medicine regulation Lox-1 and iNOS in the process of the possibility of AS mechanism.Materials and Methods: Preparation of rabbit serum Chinese medicine, by enzymatic digestion training HUVECs2-3 were used for experiment were randomly divided into 5 groups: normal control group, AngII group, low concentration of serum + AngII group of Chinese medicine, Chinese medicine in the concentration of serum + AngII group, high levels of serum + AngII group of Chinese medicine, using RT-PCR determination of human umbilical vein endothelial cell LOX-1 mRNA and iNOS mRNA.Results:1. AngII to human umbilical vein endothelial cells and Lox-1 mRNA is upregulated iNOSmRNA.2. TCM serum to human umbilical vein endothelial cells and iNOSmRNA Lox-1 mRNA expression decreased, between the low and middle dose group was statistically significant, the dose of the best, middle and high dose group had no statistical difference.Conclusion:1. AngII to human umbilical vein endothelial cells Lox-1 mRNA and iNOSmRNA upregulation.2. Expectorant medicine found the wind so that human umbilical vein endothelial cells and iNOSmRNA Lox-1 mRNA expression decreased, the dose effect of Chinese medicine in expression significant role to play good anti-AS.