| Objective:To explore the the possibility of establishment of chronic fatigue syndrome (CFS) model by single swimming,and to investigate the effects of vitamin D3on chronic fatigue syndrome and its action mechanism,and to further provide experimental basis for prevention and relieving CFS.Method:80kunming mice, weight (25to35g), were randomly divided into five groups:negative control group (C group)、Model group (CFS group) and VD groups. VD groups were further divided into bVD group, aVD group and mVD group, in which were administrated drugs1h before swimming,1h after swimming and15min after swimming respectively. Observing and recording of the body weight, feed, aggressive bite and injury observation, tail suspension test and exhaustion time to evaluat CFS Model and to explore VD3influence on CFS,while, serum testosterone and corticosterone are detected. Take tissues fo the quadriceps homogenate in mice to detect the skeletal muscle antioxidant index (T-SOD, MDA, GSH-PX, T-AOC). Skeletal muscle SIRT1, SIRT3, MEK, ERK, p-ERK protein expression were detected by Western Blot.Result:1Compared with group C, mice in CFS group manifested with loose stool, increased urination, appetite decline, poor fur burnish, nuzzles limbs, decreased activity, depression; the situation in VD3added groups is better. There were no difference in the three groups. Mice in swimming groups showed irritability, aggressive, bite each other, and with damage of ears and tail in most mice. Supplementary VD3bVD group in three groups can reduce the damage of the best effect.2Compared with CFS group, the time is significantly prolonged before mice got cardiac failure in aVD group, tail suspension time is significantly shortened, the content of blood testosterone is increased. Serum cort in grouo aVD and bVD is decreased.3Compared with group C, skeletal muscle T-SOD activity is significantly reduced, content of MAD increased, T-AOC decreaced and activity of GSH-PX reduced significantly.4Compared with group CFS, T-SOD activity in bVD and mVD increased, MDA in group aVD and bVD decreaced, GSH-PX in group bVD increased significantly.5Compared with group C, MEK1/2protein expression in group CFS increased significantly, while no difference in the expression of SIRT1, the SIRT3expression decreased obviously.6Compared with CFS group, MEK1/2expression in group aVD is significantly increased. There are significant difference in group aVD and mVD, no difference between group mVD and bVD in erkl/2expression.Conclusion:1The single swimming exercise intervention can be used to establish mice CFS model.2Supplementary VD3can alleviate symptoms of CFS at different extent, enhance the level of blood testosterone and lower levels of corticosterone, improve the symptoms of CFS in mice; Compensatory vitamin D3in three groups.The mose significant effect of alleviating symptoms of CFS was observed at the time of one hour after exercise supplement VD3.3Oxidative stress injury was observed in the skeletal muscle of the CFS model mice, and supplementary VD3at different levels can improve skeletal muscle antioxidant capacity,and the best effects were observed before exercise.4CFS can significantly reduce MEK1/2and SIRT3protein expression in skeletal muscle of the mice. Supplementing VD3at one hour after exercise rebounded MEK1/2protein expression and elevating the SIRT1protein expression. Supplementing VD3at one hour before exercise can raise the expression level of ERK1/2phosphorylation and SIRT3protein.In the three groups of mice supplemented with vitamin D3, the significant effects were observed at one hour before exercise.5Possible mechanisms of VD3to improve the symptom of the CFS is to raise the expression of cellular SIRT1and SIRT3through MEKs/ERKs SIRTl signal transduction axis. SIRT3can improve the activity of skeletal muscle antioxidant system, and play the role of the antioxidation and alleviation symptoms of CFS. The biological efffects of VD3differed significantly with different time points of VD3supplementation. |
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