Based On Metabolomics And Exercise Intervention To Explore The Metabolic Mechanism Of Chronic Fatigue Syndrome In Middle School Students

Objective:Chronic fatigue syndrome(CFS)is a kind of severe subhealth state,which is more common among middle school students,and affects their physical health and learning.But the current monitoring and diagnostic criteria for chronic fatigue are not completely established.Our team's basic research on CFS rats shows that there are metabolic disorders in the 4 metabolic pathways of CFS,including:the tricarboxylic acid cycle,glutamate,alanine and aspartate metabolism,sphingomyelin metabolism and steroid metabolism.However,at present,the metabolic mechanism of CFS is not clear.Therefore,this study combined exercise intervention to get more biometrics information,and explore the metabolic mechanism of chronic fatigue on middle school students,so as to provide experimental evidence for the prevention,diagnosis and monitoring of CFS.Methods1.According to the CDC-94 CFS criteria form the U.S.Centers for Disease Control and Prevention,5000 students from 15 middle schools in Shaanxi were screened,and selected students after the doctor5 s exclusion of other diseases.The final CFS diagnosis were screened out on 8 CFS students as CFS group,and random selected of 8 healthy students as the normal control group.All the subject's height,weight,BMI index and body fat percentage were tested.It is confirmed that there is no statistic difference before the test is started.2.Take the nails of all subjects and extract keratin.The content of 3-nitrotyrosine in keratin was determined by ultraviolet spectrophotometry.The characteristics of keratin functional groups were determined by infrared spectroscopy.3.The improved step test was carried out on two groups of middle school students to determine the index of cardiac function and the step index before and after the exercise.4.Two groups were collected urine samples before and after exercise,and urine's routine tests were performed.The metabolites in two groups of urine samples were detected by liquid chromatography-mass spectrometry(LC-MS).The main data were analyzed by principal component analysis(PCA)and orthogonal partial least squares(OPLS-DA),and the potential metabolites were searched.The biomarkers were input into MetPA database,and the corresponding metabolic pathways were analyzed.Results1.The content of 3-nitrotyrosine in keratin in group CFS was significantly higher than that in normal group(p<0.05),and there were different degrees of damage or breakage of keratin molecular functional groups' N-H bond,C-N bond,C-H bond and hydrogen bond in CFS group.2.The heart rate and systolic pressure of the CFS group were significantly higher than that in the control group(p<0.05),and the step index was significantly lower than that of the control group(p<0.05).3.The URO and urine protein of CFS group before and after exercise significantly changes compared to normal group(p<0.05).No matter before or after exercise,the differences metabolites for CFS students was creatine,its content decreased significantly before exercise,after exercise increased significantly(p<0.05);And after exercise,four new compounds:corticosterone,pantothenic acid,uric acid and xanthine metabolism,pantothenic acid and corticosterone content decreased significantly(p<0.05 or p<0.01),uric acid and xanthine content increased significantly(p<0.05).Those corresponding metabolic pathways are:arginine and proline metabolism,pantothenate and coenzyme A biosynthesis,steroid biosynthesis,caffeine metabolism and purine metabolism.Conclusion1.The results of the level of body oxidation showed that the CFS group were in a high level of oxidative stress compared with the control group.2.Under the intervention of exercise,the results of cardiac function and step index showed that the cardiac function of CFS students was lower than that of control group.3.Metabonomics analysis found that CFS symptoms may due to the body energy metabolism disorders,increased levels of oxidative stress and the hypothalamic pituitary adrenal(HPA)axis hypoactivity,involving arginine and proline metabolism,pantothenic acid and coenzyme A biosynthesis,steroid hormone biosynthesis,caffeine metabolism and purine metabolism and other metabolic pathways.