| Molecular imprinting is a technique for the preparation of synthetic polymers with predetermined selectivity for a desired template. The rigid feature of the polymer network is reflected in high chemical, mechanical, and thermal stability. The polymers show a long shelf life and can be regenerated after use. Due to their favorable molecular recognition capability and stability, potential applications of MIPs have been investigated in broad areas, such as separation, solid-phase extraction, membrane separation, sensors, et al. At present, the mole- cular imprinting technology in the pharmacy literature in the field more than half related to the separation of chiral drugs and analysis, and their active components in Chinese medicine isolation and purification of application are not many.In this study, resveratrol and phein were firstly chosen as template molecule, the single-step swelling and polymerization was developed to synthesize MIPMs in polar solvent. Here, the unique concave-dipression spatial structure has successfully been prepared using DMF as the solvent. The surface morphology of the MIPMs was characterized by scanning electron microscope. The adsorption and combine properties were characterized by IR and UV:(1) Using the polystyrene particles as seeds, adopting appropriate swelling and polymerization techniques,monodisperse MIPMs were prepared successfully by a single-step swelling and polymerization method, the microspheres were uniform and had good dispersibility with 5μm in diameter. It was excited to find that the obtained microspheres had lots of concave-dipression or cracks. The factors affecting particle size and morphology of MIPMs were studied. It was found that the different cosslinker dosage played vital role on the particle size of MIPMs. It was also found that the solvent and the swelling time played vital role on the morphology of MIPMs. Functional monomer and crosslinker were initiated and copolymerized to form MIPMs by IR spectrum.(2) Resveratrol as template molucular, DMF as Solvent, MIPMs with diameters of micrometer were prepared by a single-step swelling and polymerization method. Studied the best walnut bead preparation method, the selective binding characteristics of the template polymer were evaluated by Scatchard analysis, Study on the combination of template polymer dynamics. The results showed that: the groove surface of synthetic microspheres increased surface area and binding sites, effectively raising the rate with the combination of resveratrol and"imprinted capacity". After 30 min, the adsorption almost reached equilibrium, indicating that the imprinted cavities were saturated with the template molecules. The separation factorαwas 1.74 indicated the MIPMs exhibited specific selectivity for resveratrol. Scatchard analysis shows that the binding sites of equal class are formed in the MIPMs, and the dissociation constant Kd and maximum apparent adsorption quantity Qmax of these binding sites are 4.750 mmol/L and 95.435μmol/g,respectively.(3) MIPMs are readily obtained via the single-step swelling and polymerization using DMF as solvent, MAA as functional monomer. The preparation process was easily carried out and took little time compared with other traditional polymerization. The results show that the porous MIPMs have a high adsorption rate for template molecules because their binding sites can be accessed more easily. The adsorption kinetics was shown that the Q almost reached equilibrium after 60 min. The saturated binding capacities Qmax obtained for phein-MIPMs was 103.41μmol/g. The value of K was 0.1637 L/μmol in Langmuir adsorption analysis. The MIPMs exhibit good recognition properties for the phein. the separation factorα=1.64. |
