Micropatterning Of Two-Component Biomolecules Via UV-Induced Grafting Processes With In Situ Formed Photomask

A new method was provided for micropatterning of two-component biomolecules. The pattern of lateral bi-functional polymer brushes was prepared by photolithographic process of UV-induced grafting polymerization of poly (acrylic acid) and poly(glycidyl methacrylate) on a transparent organic film (BOPP film). The pattern of binary polymer brushes were prepared via consecutive UV-initiated grafting processes, under the assistance of the in situ formed poly (4,4'-bi['N-(4-vinylbenzyl) pyridinium]) (P(BVV)) photomask. The epoxy groups of the poly(glycidyl methacrylate) microdomains can be aminated by 1,3-diaminopropane for covalently coupling biotin with the use of N-Hydroxysuccinimide. While the poly (acrylic acid) microdomains were used for immobilizing immunoglobulin (IgG). The resulting biotin- and IgG-coupled microdomains interact specifically with their corresponding target fluorescent labeled biomolecules, FITC-avidin and Rhodamine-anti-IgG, respectively.The pattern of double polymer was characterized by Atom Force Microscopy while P(BVV) by Atom Force Microscopy and Scanning Electronic Microscopy. The picture of double protein surface with fluorescence can be captured by Fluorescence Microscope.This approach showed a way to obtain double biomolecules surface. The method, which was flexible for various biomolecules, allows the entire immobilizing patterning process to be completed using only biological buffers as solvents and across a temperature range between 25 and 37℃without subjecting either protein to ultraviolet irradiation, dehydration or harsh solvent. Compared with the traditional matrix such as SiO2 or golden film, the polymer film provided with much better flexibility and biocompatibility.