Adaptability Study Of Using Circulatory Technology Coupling Percolation And Macro-porous Resin Adsorption To Extract Different Effective Groups From Three Kinds Of Chinese Herbs

Circulatory technology coupling percolation and macro-porous resin adsorption (CTPMRA) and traditional water (ethanol) heating extraction method were applied to extract different effective groups from Salvia miltiorrhiza Bye, Radix Glycyrrhiza and Astragalus uralensis Fisch. The content and extraction ratio of the effective groups by the two methods were compared to study the adaptability of CTOMRA for extracting various effective groups of the three kinds of herbs.Water-soluble effective group salvianolic acids and lipid-soluble effective group tashinone can be extracted by CTOMRA from Salvia miltiorrhiza Bye under the conditions optimized by orthogonal design experiment, the content of salvianolic acid B (Sal. B) in salvionolic acid extract and tashinoneⅡA in tashinone extract were respectively more than 85% and 11%. The content of Sal. B in extract prepared by CTPMRA is much higher than common water heating extraction. The extraction ratio of Sal. B by CTPMRA is also very good. There are more salvianolic acid compounds with small molecular weights in the extract by common water heating extraction, which may be decomposition products of Sal. B. Room temperature extraction of CTPMRA makes the technology have advantage to extract thermal sensitive substances.Glycyrrhizic acid, Astragalus Radix polysaccharide and Astragalus Radix saponin, extracted from Radix Glycyrrhiza and Astragalus uralensis Fisch by CPTMRA technology have higher content and extraction ratio than the traditional heating extraction method. The performance of extracting flavone with CPTMRA is not very good. With the optimization of single factor experiment, the content of polysaccharide and glycyrrhizic acid in Radix Glycyrrhiza extract were more than 41% and 40%, respectively. The content of polysaccharide and saponin in Astragalus uralensis Fisch were more than 76% and 64%, respectively.There are many different effective groups in one herb medicine. The suitable extraction technology for different effective groups is different. According to the principle of circulatory technology coupling percolation and macro-porous resin adsorption, it is suitable for extracting effective groups which have good water solubility and good adsorption of macro-porous resin, especially for the effective groups which are not stable at high temperature.