Virus-free Potato Original Species Research And Application Of Cultivation Matrix

The test is divided into three parts:high temperature screening of cellulolytic bacteria and organic waste fermentation, virus-free seedlings of potato cultivation. The first part, the high temperature phase of composting from the bark of isolated cellulose decomposition ability of the two high-temperature microbial strains. On microbial growth rate, temperature resistance, the ability to size and degradation of filter paper cellulase activities were detected. The second part of the test with three factors and four levels of mixed design, test day in four phases during the fermentation substrate temperature measurement and PH, after the end of fermentation fermentation products measured bulk density, porosity, conductivity, PH value, TN, TP, TK, nitrogen, phosphorus, potassium and other physical and chemical properties. The third part dealt with through the fermentation of organic waste and other substrates for the ratio, for the cultivation of potato, growing season and harvest periods of observed and measured plant height, stem diameter, leaf number, Huanmiao speed, survival rate, By measuring the physical and chemical properties of matrix and growth of cultivated potato morphological index to filter out the original seed for cultivation of the potato-specific substrate, thus serving the potato plant production, while achieving organic waste treatment resources. The results show that:(1) The high temperature phase of composting from the bark of isolated cellulose decomposition ability of the two high-temperature microbial strains-YP and NP. Growth by strain rate, temperature resistance and the detection of cellulase activity was found, which can be 45～65℃temperature growth, optimum growth temperature was 50℃; in the general bacterial culture medium and carboxymethyl fiber Sodium (CMC) as sole carbon source medium are restricted to a fast start and strong growth, respectively, around 84h in culture up to the maximum number of viable cells. YP and NP can form in the Congo Red medium distinct brightly lit ring, its cellulase activity test results show that the isolates were obtained with a filter paper and higher Cx disintegration activity, can the 2d～4d Quick Start round filter paper decomposition,5d～8d circular filter paper strip all degraded into floc.(2) By EM, YP and NP was found that fermentation of organic wastes:fermentation strain can not change history, but you can change the duration of fermentation, the fermentation process in the PH changes during the fermentation process is a first decline in the overall curve, when Increase after reaching a lowest point process. Add the amount of acidification, whether to add more effect on the early stage of fermentation significantly, late effects are not too obvious. After each treatment with the fermentation product of physical and chemical nature of the situation, to analyze can be drawn, for the same material point of view, the size can be accumulated to a certain extent, reflect the maturity level of the fermentation substrate. Found that the temperature should not pile too high, otherwise not only inhibits the growth and activity of most microorganisms, but also the excessive consumption of organic matter, reduce the compost product quality. In which the entire fermentation process, if there is a short pre-fermentation stage of high temperature, then the fermentation would be better to complete the fermentation cycle, the time required will be shortened. At the same time come to, strains on the NP in the high-temperature properties of the best, followed by YP, fermentation end products from the considerations to YP the best, followed by EM. As for the addition of acidifier to 150g is better.(3) Virus-free seedlings of potato cultivation experiment, different treatments, the performance of plants is not the same clear differences between the different growth indicators, yield of the potato growing season after the end of significant difference. Virus-free survival rate after transplanting seedlings, Huanmiao conditions, plant height, plant stem diameter, leaf number, etc. were found, all the treatments to deal with the 8 best performance, followed by treatment for 5 and 9.(4) Physical and chemical properties through the fermentation substrate and the cultivation of comparison, the final choice of organic waste optimum fermentation conditions were:agents choose A1 (A1), Waste select B3 (sawdust), acidifier adding amount of X3 (150g /50kg).