Study On The Purification And Antioxidant Effects Of Flavonoids From Four Species Of Lilies

·Plant flavonoids have antioxidant, anti-fatigue, anti-aging and other healthcare effects. In this paper, flavonoids in the bulbs of Lilium davidii var. unicolor, Lilium lancifolium, Lilium pumilum and Lilium concolor were extracted, crude content of the extracts was determined and the purification process using macroporous resin for separation of the products from crude extracts was studied. Using rutin, catechin, quercetin and baicalein as the standard references, composition and content of purified total flavonoids extracted from lilies were detected through High Performance Liquid Chromatography. Through the establishment of in vitro oxidation system, the differences between lily total flavonoids of antioxidant and free radical scavenging capacity were investigated in order to provide the scientific basis of rational and comprehensive development and promotion of lily resources.1, Ultrasonic extraction method was used for the coarse separation of lily total flavonoids. The contents of lily total flavonoids from lily bulbs determined through spectrophotometry for L. davidii var. unicolor, L. lancifolium, L. pumilum and L. concolor were 1.65 mg/g, 2.19 mg/g, 3.61 mg/g and 4.06 mg/g respectively.2, The purification process of crude total flavonoids from lilies using D-101 macroporous resin was studied to determine the optimized technology: 1BV/h for sample flow rate, 1BV/h for elution flow rate, 3 BV for eluent consumption, 90% ethanol solution for the elution of total flavonoids from L. davidii var. unicolor, and 70% ethanol solution for L. lancifolium, L. pumilum, and L. concolor were best respectively.3, Chromatographic analysis was done to purified total flavonoids from lilies through High Performance Liquid Chromatography using rutin, catechin, quercetin and Baicalein as the standard references. Results showed that rutin, catechin and quercetin were not found in the extracts from lilies, but Baicalein only. The relative contents of Baicalein in the total flavonoids for L. davidii var. unicolor, L. lancifolium, L. pumilum and L. concolor were 54.47 mg/g, 30.82 mg/g, 22.23 mg/g and 17.95 mg/g respectively.4, Free radical scavenging ability of purified total flavonoids from lilies and rutin were investigated through the in vitro antioxidant tests. At the same concentration, the maximum scavenging rates of flavonoids extract from L. davidii var. unicolor, L. lancifolium, L. pumilum, L. concolor and rutin against O2- were 87.39%, 89.64%, 86.74%, 89.97% and 52.55% respectively, the scavenging effect on O2-: L. concolor > L. lancifolium > L. pumilum > L. davidii var. unicolor > rutin; at the same concentration, maximum scavenging rates against·OH were 25.35%, 62.83%, 75.59%, 91.78% and 35.68% respectively, the scavenging effect on·OH: L. concolor > L. pumilum > L. lancifolium > rutin > L. davidii var. unicolor; at the same concentration, the maximum scavenging rate against DPPH were 40.06%, 47.45%, 52.43%, 59.03% and 40.63% respectively, the scavenging effect on DPPH: L. concolor > L. pumilum > L. lancifolium > rutin > L. davidii var. unicolor.5, By the comprehensive analysis of total flavonoids content and free radical scavenging capacity, it was concluded that it was more significant to promote the food and medicinal value of the wild species of L. concolor, L. pumilum and L. lancifolium than that of lily cultivar of L. davidii var. unicolor in the study area of antioxidant.