The Morphology Of Theileria Luwenshuni Sporozoites From Salivary Glands Of Haemaphysalis Longicornis And Purification Of Sporozoites

Ovine and caprine theileriosis is caused by genus Theileria, which is transmitted by ticks and parasitized in macrophage, lymphblast and eryhrocytes of a variety of sheep and goats. The disease seriously inflences the sheep industry and results in many exotic sheep (or goats) and local lambs to death each year, and caused a huge economic loss of sheep industry. Theileriosis caused by Theileria luwenshuni and/or Theileria uilenbergi is an important tick-borne protozoan disease, and very common in sheep and goats in China.Investigations carried out over many years indicated that it is mainly distributed in Western China, including Gansu, Ningxia, Inner Mongolia, Qiahai, Sicuan and Shaanxi. However, the biology of Theileria sporozoite has not been systematically studied in China. In this study, Theileria luwenshuni is characterized biologically. The major results were presented below:The glands are composed of three types of acini, include typeâ… agranular acini and typesâ…¡a ndâ…¢granular acini; the typeâ… acini comprise central cell and peripheral cell at least, the typeâ…¡acini are mainly made up with a-cell, b-cell, c1-cell, c2-cell and c4-cell, the typeâ…¢acini consist of d-cell, e-cell and f-cell.Sporogony was observed only in granular acini type III and in e cells. All of the sporozoites matured when the ticks infested sheep at 6 days.A large number of sporozoites were observed in e cells, were purple blue. Specific primers amplified fragments of about 386 bp which wereconfirmed the presence of sporozoites.