Study On Wood Identification Based On DNA Technology

It is crucial to identify wood correctly for its rational and efficient utilization.The traditional morphological identification has its limitation, it is nessesary tostudy new method of wood identification, such as DNA technology. By DNAtechnological wood identification, conclusions as follow:(1) Modified SDS-CTAB method is more suitable than traditional CTABmethod and DNeasy Plant Mini Kit for the extracting of genomic DNA ofconiferous wood and rosewood.(2) Six genera of softwood which are Fir, Douglas fir, Hemlock, Spruce, Larchand Pine can be distinguished by amplifing DNA fragment of the trnL gene ofchloroplast DNA, sequencing the PCR products and sequence alignment analysis.Aquilaria sinensis and Aquilaria malaccensis which can be hardly distinguishedmorphologically can also be discriminated successfully by DNA technology.(3) The above six genera of softwood can be clusted and distinguished basedon phylogenetic tree from trnL region sequence.(4) Eight species of rosewood genus which are Dalbergia melanoxylon,Dalbergia oliveri, Dalbergia retusa, Dalbergia louvelii, Dalbergia greveana,Dalbergia cochinchinensis, Dalbergia bariensis, Dalbergia odorifera can bedistinguished by amplifing DNA fragment of the matK gene of chloroplast DNA,sequencing the PCR products and sequence alignment analysis from nestedprimers.(5) It is practical to distinguish wood among genus, possible to separate thewood species within one genus which can not be discriminated by traditionalmicroscopic features identification. It can be anticipated that molecular DNAtechnology will become an important way for wood identification, and will be apowerful accessorial tool to traditional morphological identification.