Study On Genetic Diversity Of The Plants Of The Leaf Containing Aromatic Compounds In Chimonanthus

Based on the surveyed and collected resources in the main distribution area, and the morphological and microscopic features, molecular markers and the major secondary metabolites were studied on the plants of the leaf containing aromatic compounds in Chimonanthus. The major results were as follows:1. The plants of the leaf containing aromatic compounds in C. were collected and protected, 400 germplasms of 21 populations from 6 provinces which were Anhui, Fujian, Jiangxi, Guangxi, Hunan, Zhejiang province were collected and preserved effectively.2. The classified of the plants of the leaf containing aromatic compounds in C. was confused. Through consulting the literature and specimen, investigating the morphological features in the natural resources and the garden of the plants of the leaf resources and observing the microscopic characteristics, Zhang Ruohui's view that the species containing aromatic compounds in C. were classified as C. salicifolius S. Y. Hu, C. Zhejiangensis M. C. Liu, C. nitens Oliv and C. grammatus M. C. Liu were supported and the distribution of four species were cleared. The C. salicifolius were mainly distributed to the junction area of Zhejiang, Jiangxi, Anhui provinces and the northern of Jiangxi province. The C. Zhejiangensis were mainly distributed to the junction area of Zhejiang and Fujian provinces. The C. nitens Oliv were mainly distributed to the areas of Yangshuo of Guangxi province, Xinning of Hunan province and Yichang of Hubei province. The C. grammatus were only distributed to the border region of Anyuan and Huichang of Jiangxi province.3. In use of AFLP molecular marker technique, the genetic diversity of 21 populations of the plants of the leaf containing aromatic compounds in C. was analyzed. The result showed that the plants of the leaf containing aromatic compounds in C. were low in genetic diversity. The total genetic diversity, Observed number of alleles, Effective number of alleles, Nei's genetic diversity index and Shannon's information index were respectively 0.1998, 1.992 6, 1.306 5, 0.199 2 and 0.325 1. Therefore, the protection of species resources should be more valued. Form the molecular level, the studies demonstrated the correctness of the result by Zhang Ruohui that the plants of the leaf containing aromatic compounds in C. were divided into C. salicifolius S. Y. Hu, C. Zhejiangensis M. C. Liu, C. nitens Oliv and C. grammatus M. C. Liu.4. With the material of C. salicifolius S. Y. Hu, the study on the chemical compositions of the plants of the leaf containing aromatic compounds in C. were conducted preliminarily. The results showed that there were rich volatile and non-volatile substances in these plants. The volatile compounds were mainly eucalyptol, borneol, bornyl acetate, furan, (E)-3-(4,8-dimethyl-3,7-nonadienyl)-, and (E,E)-Farnesol etc. The non-volatile substances were scopoletin (1), scopoletin-7-O-β-D-glucoside (2), kaempferol-3-O-β-D-glucoside (3), kaempferol-3-O-β-D-rutinoside (4), kaempferol (5), quercetin (6), and Emodin-8-O-β-D-glucopyranoside (7). The compounds 1, 5 and 6 showed more obvious antioxidant activity than positive control Trolox with IC₅₀ values of 8.72 mmol·L^-1,1.53 mmol·L^-1 and 0.56 mmol·L^-1 respectively.