54 Guangxi Regional Trial Sugarcane Varieties Of The Drought And Drought Resistance Gene Analysis Of The Separation

This research was divided into two parts:Firstly,54 sugarcane varieties were studied at the seeding stage under natural drought stress in order to explore the changes of the physiology and biochemistry indexes of the +1 leaves like relative content of chlorophyll, relative water content,leaf water potential, SOD/POD activity, the content of MDA or pro and so on. The membership function and principal constituent analysis methods were adopted to estimate the ability of drought resistance of the 54 sugarcane varieties under the water recovery condition. And the result showed that they were divided into three groups:highly drought resistance, moderate drought resistance and the disability of drought resistance.There are 13 highly drought resistance varieties, GT467,GT1247,GT02-235,ROC22,GT496,YT57-423,G28,NCO310,GT1156,GT649,ROC10,GT97-69,GT2357.20 moderate drought resistance varieties are:GT3,GT21,GT99-107,GT02-761,GT2625,GT02-899,GZ18,YL17,GT208,GT97-40,GT963,GT342,GT1237,GT02-770,L03-1137,B1,GT02-833,F172,G17,B8。And the disability drought resistance varieties are:YT93-159,GT02-281,YL6,B28,GT02-413,GT02-901,GT02-351,L04-12,GT123,GT11,ROC16,GT133,L03-182,GT99-181,L03-255,GT619,GT02-237,GT390,GT02-469,GT1403,GT02-99.Secondly, RNA was extracted from the highly drought resistance variety ROC22 and the disability drought resistance variety ROC 16 which were treated under water stress at the elongation stage. And reverse transcription was carried out to obtain cDNA template, then adopted the Sequence-related amplified polymorphism (SRAP) marker to sift out the drought resistance gene with 108 pairs of SRAP primers. And the result showed that:The best amplification system and conditions of sugarcane SRAP mark were set up which were do a great favor to separate more drought resistance.11 differential sequences were sifted from the ROC22 and ROC 16 with the 108 pairs of SRAP primers. And one Pyrroline-5-carboxylate dehydrogenase differential sequence was obtained after re-amplification, purification and sequencing.