Establishment Of Regenerationg System Of Pendent Cymbidium

In this study, the seeds and lateral buds of pendent Cymbidium were used as a source of initial explants for rapid propagation in vitro. The suitable medium formulas were screened including induction, proliferation and differentiation, plantlet rooting and transplanting.Finally the regeneration system of pendent Cymbidium was established.The results were as follows:1,The establishment of sterile germination technology system.The best way of sterilizing capsule was as follows: it was dipped in 72% ethanol for 1 min, then burned by the alcohol lamp two times after dipped in 95% ethanol.The best medium for seeds induction was 1/2MS+6- BA 1.0mg/L + NAA 1.0mg/L + AC 0.5g/L. The condition of liquid shaking culture was better due to its conductive to seed germination.The best medium for PLBs proliferation was 1/2MS+6-BA1.0mg/L +NAA 0.5mg/L +KT 1.0 mg/L + CM 100g/L +AC 0.5g/L. The inoculation density and culture methods had greater impacted proliferation. 10 PLBS and liquid shaking culture was conductive to buds proliferation.The best medium for PLBs differentiation was 1/2MS+6-BA 0.5 mg/L +NAA 0.1 mg/L+ AC 0.5 g/L.The best medium for rooting and seedling cultivation was 1/2MS+IBA1mg/L+ mashed potatoes 50g/L.2,The establishment of Buds means tissue culture system. The suitable methods of sterilizing of lateral buds: it was dipped in 72% ethyl alcohol for 30s, then agitating in a solution of 0.1% HgCl2 for 8 min, and rinsed three-four times with sterile distilled water, and stripped out 1-2 leaves, and further agitating in a solution of 15% sodium hypochlorite for 4 min.The best medium for buds induction was 1/2MS+ 6-BA 4.0 mg/L + NAA 0.2 mg/L + AC 0.5 g/L.The best medium for buds proliferation was 1/2MS+6-BA 4.0 mg/L + NAA 0.1 mg/L + AC 0.5g/L.The best medium for buds root and seedling cultivation was 1/2MS + NAA 2.0mg/L+ banana 50g/L+AC 1g/L.The best planting medum was moss. The survival rate of transplanted plantlets was up to 100%.