Diversity Of Endophytic Microbial Community Of The Populus Euphratica In Lopnur County

It investigated the diversity of the endophytic microbial community thatassociated in the storage liquid in stems of the Populus euphratica in Lopnur County,Xinjiang by the culture-independent method.Total community DNA was directlyextracted from Populus storage liquid of the Populus euphratica. The clone library ofribosomal RNA genes was amplified using PCR with universal primer sets of bacteria,archaea and fungi. And then bacterial, archaeal and fungus clone libraries of the rRNAgene were constructed. The results were the following:1. For the bacterial clonelibrary,400bacterial clones were randomly selected, which could be grouped into48ribotypes, based on restriction fragment length polymorphism (RFLP) analysis.Phylogenetic analysis showed that48Operational Taxonomic Units (OTUs) belongedto Firmicutes, Actinobacteria, Proteobacteria (Alpha-, Beta-, andGamma-proteobacteria), Bacteroidetes and Verrucomicrobia,respectively. Of them,Gamma-proteobacteria were the most dominant group, representing53%of the totalbacterial clone library.2. For the archaeal clone library,52positive clones wereselected and4ribotypes were determined, phylogenetic analysis indicated that allribotypes were affiliated with the Methanoculleus of phylum Euryarchaeota. Thefungus clone library was amplified using PCR with two pairs of universal primer.3.For the fungus18S rRNA gene clone library, a total of13OTUs were only5OUTsattributable to the fungal,8OUTs attributed to eukaryotic Chromalveolata andRhizaria. For the fungus ITS rRNA gene clone library,148positive clones wereselected and8ribotypes were determined, phylogenetic analysis indicated that allribotypes were affiliated with the phylum Ascomycota, and98%of clones belongedto Uncultured fungus clones.The obtained data demonstrated that bacteria diversity in the storage liquid of thePopulus euphratica was abundant, while the archaeal and fungi community structurewere unitary. The study was the first use of culture-independent method for understanding the microbial community structures and diversity in the plant samples,and the results would provide some therotical bases for isolation and pure cultivationof endophytic microbial resource in the Populus euphratica.