Effects Of N-Acetylcysteine On The Pulmonary Fibrosis Of Chronic Ethanol Ingestion Rats

Objective:The drinking liquor can injure many organs of human being. Alcohol on human being has potential biological effects in almost every organ system. Drinking liquor can lead to alcoholic Wernicke disease,alcoholic liver cirrhosis, alcoholic subacute combined degeneration, alcoholic cardiomyopathy, learning and memory abilities declining, alcoholic Osteonecrosis,accidental injury, and so on. The impact of alcohol on the lung have a great clinical importance, its volatility makes airway epithelium on high density ethanol surroundings. It can cause airway epithelial injury and lung tissue remodeling, and lead to alcohol-related pulmonary disease. At present, the research about alcohol-induced pulmonary fibrosis is little, so the study aims to set up the model of chronic ethanol ingestion induced pulmonary fibrosis in rats, and explore the relationship between alcohol and pulmonary fibrosis and it's possible mechanism for the pathogenesis of pulmonary fibrosis with detecting the content of SOD, MDA of lung tissue. It is significant to find the incidence of pulmonary fibrosis, at the same time to have further understanding the harm of chronic alcohol ingestion.Methods:30 Clean healthy male Sprague-Dawley rats, weighing 160-200g, were randomly divided into alcohol group(10), alcohol+N-Acetylcysteine(NAC) group(10), and control group (10). Feeding quantitative non-ethanol or ethanol Lieber-DeCarli liquid diet every day in a single cage for 8 weeks. Alcohol+NAC group were given NAC 300mg/kg/d. At study completion, animals were sacrificed, The morphological changes of lung tissue were observed by hematoxylin-eosin staining, Interstitial lung collagen deposition were observed by Masson staining; The activity of SOD and content of MDA of lung tissue were detected. SPSS13.0 statistical software was used for data processing, The data was analyzed with one-way ANOVA. The level of significance was set as P<0.05.Results:1.The general status:There were droop, gloomy, moved slowly, reacted slowly Irritable, soft row of faceless stool in rats of alcohol group, the rats of alcohol+NAC group also appeared the similar behavior, however the degree was lower than alcohol group, the behavior of control group was well:their behavior being vivid, their reaction being agile, and their hair being slick.2. pathological results:there were varying degree of alveolar and alveolar septal infiltration of inflammatory cells, some alveolar wall damaged or collapsed in Alcohol group, and there were more deposition of collagen fibers at intervals of alveolar in Alcohol group. The similar presents were in alcohol+NAC group, the degree was lower than alcohol group. the control group alveolar structure was clear, and alveolar walls interstitial have not inflammatory cell infiltrations. The degree of alveolitis and the degree of pulmonary fibrosis in alcohol group were higher than it in control group (P<0.01), The degree of alveolitis and the degree of pulmonary fibrosis in alcohol group were higher than it in control group, (P<0.01; P<0.05, respectively).3. the SOD activity of lung tissue in alcohol group was lower than control group, the MDA content of lung tissue in alcohol group was higher than control group, differents were statistically significant(P<0.01). the SOD activity of lung tissue in alcohol+NAC group was higher than alcohol group, the MDA content of lung tissue in alcohol+NAC group was lower than alcohol group, differents were statistically significant (P<0.05).Conclusion:This experiment proved Lieber-DeCarli Ethanol liquid diet can establish chronic ethanol ingestion rats' animal model. Alcohol can induce oxidized stress response; Oxidized stress products make rat lung tissue expression increase, incude pulmonary fibrosis. And NAC can increase the activity of SOD of the lung tissue, decrease the MDA content, reduce the degree of pulmonary fibrosis. It was proved that alcohol play an important role on the formation of pulmonary fibrosis, suggesting that chronic drinking can cause lung injury in rats, leading to pulmonary fibrosis.Alcohol is one of the causes of pulmonary fibrosis. NAC can raise the SOD activity and decrease the content of MDA of lung tissue in chronic ethanol ingestion rats, restrain the oxidative stress induced by alcohol. NAC can provent lung tissue from pulmonary fibrosis incuded by alcohol.