Agastache Rugosa And Dried Tangerine Peel Cooperatively Regulate The Gastrointestinal Motility And The Best Ratio Of Their Medicinal Compatibility

Objective:Gastrointestinal motility function is one of the most important physiological functions of the digestive system.Whether is it normal or not, depending on the central nervous system, the enteric nervous system, gastrointestinal hormones, gastrointestinal electrical rhythm and so on. The normal gastrointestinal motility is not only pushing, grinding, and mixing, but also having immune regulation, hormone secretion and mucosal barrier such as the role of non-specific immune defense. It is known that the patient would often have abdominal distention, abdominal pain, nausea, vomiting, gastrointestinal motility disorder and a series of performance because of suffering from trauma, surgery, radiotherapy, chemotherapy or severe infections.Therefore it is essential to use drugs early to restore function of gastrointestinal tract disease recovery and prevention of complications. Nowadays, researehs have found that there are many factors could generate gastrointestinal motility disorder and the modern medicine try to develop new drugs to promote gastrointestinal motility,but most of them have many side effects. However, Chinese medicine have a good clinical effect on treatment gastrointestinal diseases.Modem medicine's understanding of gastrointestinal digestion and absorbability equal with the function of the spleen and stomach in the traditional Chinese medicine. The movement of Qi-lifting power and stomach movement theory are accordant. The symptoms of gastrointestinal motility disorder are attributable to the performance of "Piman",which means that the occurrence of stomach fullness Qi-block, directly related to abnormal movements, It is that the similarities and relevant between Chinese medicine Piman and Western medicine FD that prompted the scientific hypothesis and ideas for the Chinese medicine treatment of these diseases.Nowadays,it has been reported that the researehs on the aromatic Qi medicine and regulate Qi medicine regulating gastrointestinal motility. However, it has not been reported that the researehs on the aromatic Qi method and regulate Qi method regulating gastrointestinal motility. Especially, the proportion of drug compatibility changes in dose and Pharmacodynamic Study of GI Dynamics is still rarely reported.TCM holds that:the reason of the disorders of spleen and stomach is mainly because of the disorder of Qi.Therefor,it is necessary to use regulate-Qi method and aromatic-Qi method. Early researchs on gastrointestinal motility around the aromatic effects of pharmacological studies have shown that the pathogenesis of aromatic herbs on intestinal mucosal injury in diverse non-specific protection, gastric mucosal blood flow can be increased.finally intestinal peristalsis excited and the gastrointestinal propulsive movement accelerated.For example, orange peel, costustoot and official magnoliaoody have the bidirectional regulation of gastrointestinal smooth muscleIt is impoint that focus on studing group prescription drugs combination reasonation,including compatibility between the characteristics of medical treatment and prescriptions of the core.TCM treat the Piman using compound herb to complete the on gastrointestinal motility and antagonized the synergistic effect of multi-target adjustment.The subject of the preferred combined aromatic Qi law with regulate Qi law.Select the represent of drugs, agastache and tangerine peel.The use of in vitro and in vivo model to investigate the aromatic Qi and regulate Qi in the compatibility and the combination of FD rats intervention of gastrointestinal motility disorders, the mechanism of coordinated intervention, We finally complete the study on agastache rugosa and dried Tangerine peel cooperatively regulate the gastrointestinal motility and the best ratio of their medicinal compatibility.Methods:1 Limb ischemia-reperfusion model was used to simule FD, the establishment of Agastache rugosa and dried tangerine peel with same dose of single agent and combined product decoction combined with different ratio of the observation group.2 Preparation of FD rat gastric longitudinal smooth muscle strips, by observing the contraction of gastric smooth muscle the mean amplitude of change, Agastache rugosa alone boiled with dried tangerine peel, the same dose combined decoction of FD intervention in gastric smooth muscle contraction role and mechanism of coordinated intervention.3 Carbon powder of marching method, observing residual rate of the rat stomach and small intestine to promote the rate of change, analysis and comparison with aromatic Qi under the same drngs combined ratio of compatibility, in the efficacy of intervention in the FD role of gastrointestinal motility differences and coordination with dried tangerine peel and Agastache rugosa adjusted to optimize compatibility proportion of gastrointestinal motility.4 By radioimmunoassay techniques, observing the proposed FD rats of the blood motilin and gastrin levels, target confirmation from the gastrointestinal hormones, Agastache Combinatorial Optimization compatibility with dried tangerine peel ratio of compatibility. Compatibility with orange peel and Agastache rugosa and combination of regulation of gastrointestinal motility in FD, and promote the mechanism of gastrointestinal motility.Results:1 Use limb-reperfusion (LIR) model successfully reproduce the stress state of the gastrointestinal motility disorder (FD). FD ischemia 3h,12h after reperfusion, mean contractile amplitude of gastric smooth muscle was significantly decreased (P<0.05), gastric pathology seen oozing mucosal stroma, congestion, edema, and to see a small neutrophil cell infiltration.2 Different doses of Agastache rugosa and dried tangerine peel with the same single dose of co-decoction could significantly increase the FD rat gastric longitudinal smooth muscle contraction mean amplitude (P<0.05), promotion of gastrointestinal smooth muscle contraction. Among them. Agastache high dose (100mg/ml), dried tangerine peel, the low dose (50mg/mI,25mg/ml), Agastache+ the same low-dose combined decoction of dried tangerine peel (12.5+12.5 mg/ml) the role of the best.3 Combined decoction of compatibility ratio of FD can be effectively coordinated intervention to promote gastrointestinal and gastrin secretion. Effects were markedly sex, Agastache+dried tangerine peel (0.4+0.3) g/ml> Agastache+ tangerine peel (0.4+0.2) g/ml>Hong+tangerine peel (0.3+0.3) g/ml dose combination. Agastache+ tangerine peel (0.3+0.2)g/ml dose of the combination of FD effects of gastrointestinal motility without intervention.Conclusion:Agastache rugosa and dried tangerine peel extract single treat FD rats by enhancing the longitudinal strips of gastric smooth muscle contraction amplitude, contraction of gastric smooth muscle. Among them, high doses of Agastache rugosa, tangerine peel, the most significant effect of low doses; the same dose of low dose combined decoction in FD intervention effect due to the best of gastric smooth muscle relaxation. Thus Tips:Agastache+orange peel effect on gastric smooth muscle positive adjustment has good synergy; rugosa decoction combined with dried orange peel enhance the gastrointestinal propulsive role, and participation is closely related to gastrointestinal hormones. Agastache proportion of compatibility with the orange peel effect to play a key factor in synergy. Confirmed:aromatic Qi method superimposed with the application of regulate Qi method, the best dosage ratio of 4:3 and 4:2. Aromatic drugs dosage> regulate Qi drugs, enhance synergies. Otherwise, synergies would be weak, even antagonistic.