Anti-cancer Effect And The Influence Of The Cell Growth Cycle On S180 Mouse Treated By Yiliuning

Purpose:Nowadays malignant tumour has become common disease and frequently-occurring disease threatening human health. We have gone through a long process to explore the therapy of malignant tumour. Previously, people believed that surgical operation should follow monoblock and widely radical operation principle in Western medicine, considering that the wider it was excised, the more thorough it was cured. At present, people's attitudes toward oncotherapy turns to preserve tissues and functions. Consequently,multiple treatment options get developed,such as individual operation regimen,minimally invasive surgery,transplant repair surgery and preventive surgery.Medicine also transforms from researching and developing kinds of relatively non-specific cytotoxic agents used for taking turns killing and wounding cancer cells of different cell cycle phases since nitroqen mustard came out to targeted therapy,including monoclonal antibody,tumor vaccine,Antiangiogenic drugs,Antioxidant cytotoxic drugs,Inducing tumor cell differentiation apoptosis,Resistance to signal transduction treatment,gene therapy etc. Radiotherapy is no exception, shifts from applying single ionizing radiation causing multicellular damage to combined therapy which makes its adaptability increase, curative effect improve and meanwhile reduces the damage to the normal tissues. The traditional Chinese medicine also converts from the view of strengthening the body resistance, that is improving prognosis, to the direction of eliminating pathogens by inhibiting tumor cells growth and inducing them apoptosis in the treatment of malignant tumor. Chinese herbal medicines has inneglectable advantages such as low cost and small toxicity etc. Yiliuning is deemed as a kind of antineoplastic prescription on that basis.There are spectacular results in the therapy of digestive system neoplasms applying Chinese herbal medicines,especially esophageal cancinoma, gastrocardiac carcinoma,liver cancer, pancreatic cancer etc. Meanwhile, they have definite curative effect and neither obvious toxicity of liver, kidney and heart nor bone marrow inhibition for a long-term use. Combined with chemotherapy, they can add curative efficacy, reduce side effects and so on.Yiliuning is composed of Rab,Aca, Duc and Sol.The experiment in vitro indicated that it could obviously inhibit the growth of diversified tumor cells,including esophageal cancer Eca-109 cells,gastric cancer SGC-7901 cells,pancreatic cancer BBxpc-3 cells,and liver cancer Bel-7402 cells,breast cancer MDA-MB-231 cells,lung cancer NCI-H520,lung cancer A549 cells,cervical cancer Hela cells. The experiments in vivo demonstrated that it had an anti-growth effect on S180 solid tumor mice and prolonged survival of mice with S180 ascites tumor.The anti-tumor mechanism might be TOPO-â…¡(the down-regulation of topology isomerase-â…¡) expression.It had an evident anti-tumor effect on tumor-bearing Ec-9706 nude mice.Influencing the expression of within an enzyme called cox-2 (cyclooxygenase-2) and ki-67 (the nucleus related antigen) in cancer cells,it could inhibit tumor angiogenesis and tumor growth.We observed the acute toxicity of Yiliuning and the test on S180 solid tumor mice and ascites tumor mice, then analyzed the influence of the cell growth cycle, which provided experiment evidence for clinic.Methods:Acute Toxicity Test:Infuse liquid medicine into mice's stomach by 500g/kg(the minimum no lethal dose),250g/kg,167g/kg,125g/k,100g/kg(the most live dose)until all survive,each dose group of animals is 6 (bisexual each half),and then observe drug toxicity two weeks. Official Test:According to the result (100g/kg)of the previous experiment, observe drug toxicity two weeks after twenty mice(bisexual each half) are perfused.The pharmacodynamics Test:Establish the model of solid tumor and ascitic tumor male mice S180,and then random separate into five groups, eight each group: Negative control group(the same volume of physiological saline); Positive control group (solid tumor:20mg/kg.d of the cyclophosphamide,ig;ascitic tumor:0.2mg/kg.d of the mitomycin,ip);low,moderate,high three dosage groups of Yiliuning(7.47g/kg.d, 14.94 g/kg.d,22.41g/kg.d of Yiliuning'extracts, respectively,ig). The administration route is gavage through mouth in all the groups. After giving drugs for ten days continuously,observe the changes of tumors'weight and generally of mice in each group. On the 11th day, execute all the mice of solid tumor groups, take out the neoplasms to weigh, calculate the tumor-suppressing rates To ascitic tumor,After giving drugs for ten days continuously,observe the groups of mice survival time.and calculate the life-prolonging rate.The experimental mechanism:Making cell suspension to the tumour. Then analyse the content distribution of DNA using FCM and the propotion of cells of G1, G2,S phases via multicycle software.Results:T Acute toxicity results of Yiliuning is 100 g/kg. which is as much as 120.5 times of clinical medication.The tumor-suppressing rates in positive control group and experimental groups (low,moderate, high three dosage-groups of Yiliuning) are respectivly 64.15%, 33.49%,35.38%,39.62%.Compared with negative control group,the difference of all groups are statistically significant(P<0.05).As the dosage increases,the effect of anti-tumor is more and more obvious among the Yiliuning groups,which shows a quantity-efficiency relation. When the experiments are over,the weight of the mice in the positive group and Yiliuning group are higher than that of the negative control group. Yiliuning group with high dosage is 23.53%. Compared with the negative control group,the datas have statistically significant difference,but have no practical significance. The life-prolonging rate of the Yiliuning group with low, moderate dosage are respectively 6.86% and 14.71%,and there are no statistically significant difference(P>0.05) compared with the negative control group. The S phase rates in experimental groups (low,moderate, high three dosage-groups of Yiliuning) are respectivly 52.226%,58.206%,59.004%.Compared with negative control group,the difference of all groups are statistically significant(P<0.05).Conclusion:1.During observation period,there are no death animals and do not find toxic effects in short-term after giving 100g/kg of Yiliuning;2.The compound preparation of Yiliuning can obviously restrain the growth of solid tumor Kunming mice S180, which shows a quantity-efficiency relation;3. Compared with control group, the high dose group, which have statistically significant difference,but have no practical significance.4.The anti-tumor mechanism of the compound preparation of Yiliuning may be inducing tumor cells apoptosis, and blocking tumor cells in S phase.