The Research Of Anti-cancer Effects And Cells Kinetics On S-180Tumor-bearing Mices By Yiliuning And Its Components’ Extracts

Objective:Nowadays, cancer is a serious hazard to human life and health of major diseases,its incidence increased year by year, so the cancer has attracted worldwide attention of the scientists,the study of cancer involves a lot of fields,for example:cancer etiology,the formation of grug resistance,metastasis,the killing effect of anti-cancer medicine on malignant tumor cell and so on. but the occurrence of cancer is a very complex process, so far we have not yet to clear the causes, and therefore we have not found a satisfactory way to cure cancer,what we to do is prolong the survival timt of patients,improve the life quality,reduce the damages of cancer to human body.Therefore we still in the exploring stage of oncology.thre are many problems waiting to be solved.Surgical resection is the treatment of choice for eraly stage or non-metastasis.according to the pathology and postoperative phase to determine whether use adjuvant therapy;For the patients with advanced malignant tumors,comprehensive treatment is the main method.we can choose chemotherapy combined with radiotherapy.The patient who suitable for TKI,the patient can choose accord to their economic situation. CIK cells biological treatment is also a good way treatment.However the traditional methords of treatment have greater impact on human body,such as after esophagectomy,the anatomic change of esophagus may lead to some influence on gastrointestinal function;chemotherapeutic drugs has the ability of killing cancer cells,but at the same time it can kill the normal immune cells and weaken our immunoty,so the cancer cell have the chance to grow and metastasis.TKI and CIK biological therapy,for the reason of their high expensive,so under the existing conditions of our country can not be universal.Chinese medicine is a great treasure. In the ancient China have the record of using chinese medicine to treat the cancer.Because of its efficacy,low toxicity and inexpensive price,chinese medicine paly a great role in the comprehensive treatment of malignant tumors.Yiliuning is a traditional Chinese compound prescription composed of Rab,Aca,Duc and Sol,which is a clinical experienced prescripsion of our technical office.It has definite affirmative effect on the treatment of tumours for digestive system,and has little toxicity.According to our former experimental in vitro anti-tumor and result of its four compositions’extracts which compound with Rab,Aca,Duc and Sol can control growth and muitiplication of tumor cells;induce tumor cells to apoptosis and arrest the Eca-109cells during S-phase,the mouse’s acute toxicity experiments have shown when maximatily dosage is126times than clinical, acute toxic reaction does not appear.Yiliuning have no obviously toxicity in short time,and the pharmacodynamics experiment has confirmed that tumor inhibition rate of Yiliuning were greater than30%to H-22and S-180. and can extend survival days of leukemia L1210tumor-bearing mice in a short time.The objectives of this experiment are to observe the effect of Yiliuning’s anti-tumor and potential cell kinetics mechanism of action on S-180tumor-bearing mouse, provide experiment basis for the use of clinic.METHORD:The experiment of antitumor on S-180tumor-bear mice and cell kinetics.The model of tumor-bearing mice were established by S-180fibrosarcoma cells.the mice were randomly divided into two big groups.The first group:model control group(CMC2ml/10g ig10days);Positive control group(0.2%cyclophosphamidum20mg/kg ip10days);Low-does Yiliuning group:crude drugs7.74g/kg,middle-dose group:crude drug14.94g/kg,high-dose group:crude drug22.41g/kg;The second grooup:model control group(CMC2m1/10g ig10days);Positive control group(0.2%cyclophosphamidum20mg/kg iplOdays),Low-dose Rabdosiarubescens group:crude drug2.25g/kg,middle-dose group:crude drug4.5g/kg,high-dose group:crude drug6.75g/kg.Low-dose Duchesnea group:crude drug1.5g/kg,middle-dose group:crude drug3g/kg,high-dose group4.5g/kg;Low-dose Acanthopanax group:crude drug2.25g/kg,middle-dose group:crude drug4.5g/kg,high-dose group:crude drug6.75g/kg.Low-dose Solanum nigrum group:crude drug0.75g/kg,middle-dose group:1.5g/kg,high-dose group:crude drug2.25g/kg.The experimental group mice were the drug via mouth for ten days.The weight of the mice and tumor change、 general situation were observed from the first day,kill the mice at the11th day,weigh the tumor that removed from the mice and calculated the rate of inhibiting tumor.At the same time,the tumor were made into unicellular suspension,then check the proportion of each cell cycle by flow cytometry.Results:The Inhibition effects of Yiliuning and iits prescription extracts on S-180tumor-bearing mice:The tumor inhibition rate of positive control group is58.13%,The tumor inhibition rate of Yiliuning low-dose group、 middle-dose group、 high-dose group is38.61%、41.86%、42.27%. The second grouup:The tumor inhibition rate of positive control group is55.32%,The tumor inhibition rate of Rabdosia rubescens low-dose group、middle-dose group、 high-dose group is32.11%、36.59%、38.57%. The tumor inhibition rate of Duchesnea low-dose group、 middle-dose group、 high-dose group is31.30%、36.17%、40.24%. The tumor inhibition rate of Acanthopanax low-dose group、 middle-dose group、 high-dose group is32.92%、34.95%、39.02%. The tumor inhibition rate of Solanum nigrum low-dose group、 middle-dose group、 high-dose group is28.05%、30.89%、36.18%.Compared with the control group statiscally significant difference(P<0.05).The result of flow cytometry analysis:Yiliuning can arrested the S-180cells in S phase. Rabdosia rubescens can arrested the S-180cells in G2/M phase. Duchesnea can arrested the S-180cells in G0/G1phase. Acanthopanax can arrested the S-180cells in G2/M phase. Solanum nigrum can arrested the S-180cells in S phase. Conclusion:1. The extract of Yiliuning and its components can improve the general situation of S-180tumor-bearing mice.2. The extract of Yiliuning and its components can obviously resist the growth of tumor,and the tumor inhibitory rate of YILIUNING is better than extracts of the components of YILIUNING.3. The anti-tumor mechanism of Yiliuning and its components extract may block one of a cell cycle,achieve the purpose of inhibiting tumor growth.