| Objective:This study using somatosensory evoked potential test and double immunofluorescence staining technique to observe the vibration method of cerebral ischemia and early somatosensory evoked potential structural plasticity indicators-growth associated protein and synaptophysin explore the effect of vibration treatment of brain missing the validity of the blood of stroke, and judge its efficacy, so as to massage the treatment of acute cerebral ischemia and provide a theoretical basis.Method:Choose healthy male SD rats of clean grade 40, weight 300±20g, were randomly divided into four groups:control group, model group, model+EA treatment group, model+ vibration treatment group. One control group without any intervention, the model group and treatment group were middle cerebral artery occlusion (MCAO) model of focal cerebral ischemia is made. Successful model in the treatment group, the day after the EA respectively treatments with the vibration method, select Baihui, electro-acupuncture treatment group, EA treatment group, Han acupoint nerve stimulator (HANS-LH202H), using density wave, frequency 2Hz/15Hz, intensity of 1mA, strength to shake slightly as the degree of rat head, duration 20 minutes, day 1 for 14 days. Vibrating massage therapy group received treatment for the vibration stimulus, the output peak voltage 4~6V, stimulation frequency 50Hz, the duration of 20 minutes, day 1, continuous 14d. The group were taken at day 13, the first 7 days to take three, take four 14 days, after the end of treatment, testing SEP, after testing detected by immunohistochemistry growth associated protein-43 (GAP-43) and synaptophysin (SYP) observation of dynamic expression, and to do statistical analysis.Results:l.The rats at different time of somatosensory evoked potential P1 wave amplitude comparison:①After model day 1, blank control group and model group were significantly different (P <0.01); control group and EA, vibration method group were significantly different amplitude (P<0.05); model group and EA group amplitude vibration method were significantly different (P<0.05). ②At the 7 days, model group and the other three groups had significant differences in amplitude and latency (P<0.05); but the control group andEA, vibration method was no significant difference (P> 0.05).③At the 14 days, the amplitude and latency between the groups was no significant difference (P> 0.05).④In each period, the EA group compared with the vibration method was no significant difference (P> 0.05).2. The rats at different time of somatosensory evoked potential latency comparison of P1:①After model day 1, control group and the other three groups of amplitude and latency were significantly different (P<0.05).②At the 7 days, model group and the other three groups had significant differences in amplitude and latency (P<0.05); but the control group at EA, vibration method was no significant difference (P> 0.05).③At the 14 days, the amplitude and latency between the groups was no significant difference (P> 0.05).④In each period, the EA group compared with the vibration method was no significant difference (P> 0.05).3. The rats at different times of growth associated protein-43 (GAP-43) expression in comparison:①in modeling after day 1, blank control group and the other three groups were significantly different (P<0.01), model group and the EA group, vibration method was no significant difference (P> 0.05);②At the 7 days, the model group and control group were significantly different (P＜0.01), model group and the EA group, vibration method groups were significantly different (P <0.05), control group and the EA group, vibration method groups were significantly different (P<0.05);③At the 14 days, the model group and the other three groups were significantly different (P <0.05), blank control group and the EA group, vibration method were no significant differences (P> 0.05);④at all times, the electric acupuncture group compared with the vibration method was no significant difference (P> 0.05).4. The rats at different time synaptophysin (SYP) compared the expression:①In 1 day after modeling, the control group and the other three groups were significantly different (P<0.05), model group and EA group, vibration method was no significant difference (P> 0.05);②at the 7 days, control group and the EA group, vibration method was no significant difference (P> 0.05), model group and the other three groups were significantly different (P <0.01)；③At the 14 days, blank control group and the EA group, vibration method groups were significantly different (P<0.05), model group and the EA group, vibration method groups were significantly different (P<0.05); blank control group and model group no significant difference (P> 0.05);④n each period, the EA group compared with the vibration method was no significant difference (P> 0.05).Conclusion:After treatment can be found on the one hand, vibration method can not only speed up the rat somatosensory evoked potential recovery of PI wave amplitude, but also can shorten the latency of the induced time, further evidence of the somatosensory evoked potential (SEP) experiment in the test phase of cerebral ischemia and recovery application reliability and validity. On the other hand, the growth of cerebral ischemia associated protein-43 (GAP-43) and synaptophysin (SYP) expression were significantly restored, close to the normal range, compared with the model group significantly different, indicating that needle sting and vibration method can speed up the early brain plasticity. Vibration method in this study group and the acupuncture treatment group showed no significant difference. |
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