| Although the spinal cord blood stream was resumed from tissue injury byseveral causes,nerve function did not been improved but aggravated ,whichresult in ischemia reperfusion(I/R) in spinal cord。The immune system play a vital role on spinal cord I/R,inflammationcells were activated ,cytokines were released,which result in spinal cordinjury.Therefor, inhibiting immune response is a important strategy for treatspinal cord injury during I/R.IL-10 is a anti–inflammation cytokine,which can inhibit the produce ofsome inflammation medium and cytokines.IL-10 become interesting in thestudy of ischemia reperfusion. But it was not reported in the filed of spinalcord I/R .Therefor the study aim is to investigate the effect of IL-10 on spinalcord I/R, so that offer a new method for preventing and treating spinal cordI/R.To set-up rat animal model of spinal cord I/R by occlusion of theabdominal aorta producing lumbosacral spinal cord ischemia . Abdominalaorta was obstructed and opened according to events ofischemia-reperfusion.Laser-Doppler flowmetry was employed to measurehemodynamic changes in the oumbal spinal cord.To gather the spinal cordtissue and serum according to preischemia,30min of ischemia ,60min ofischemia,and 0.5h,2h,8h,16h and 24h of reperfusion respectively. Spinal cordtissue and serum of IL-10 was detect separately by RT-PCR and ELISA.Spinal cord tissue MPO activity was mensurated as to estimate neutrophilrecruitment.The examination of spinal cord tissue histopathology through HEstaining and Niss body staining .The result show spinal cord tissue and serum of IL-10 expression wereincreased after I/R 8h.At I/R 2h,there was not significant different frompreischemia and ischemia group.MPO activity arise in I/R 0.5h,the increaserate was lowwed after I/R 8 h. Spinal cord tissue histopathology change isaggravated along with I/R progress.These results illuminate IL-10 wasexpression during spinal cord I/R,but its expression is late than MPOactivity increase. Here is a body protect responses against spinal cord I/Rinjury.At the base of above study, we investigated the effect of exogenousIL-10 on the protect for spinal cord injury and the change of TNF-a andICAM-1 during spinal cord.To set-up rat model of spinal cord I/R as above .There are two ways toinject IL-10.One way is that IL-10 by s.c (5ug/kg)before 1h of I/R .Otherway is that inject IL-10 by i.v (5ug/kg)after 30min of I/R.The two way haveseparately role of prevent and treatment. spinal cord I/R. To gather tissue andserum at 8h, 48h of reperfusion respectively. Apply Tarlov criterion score toevaluate hindlimb neurologic function . Estimating spinal cord tissue injuryuse Niss body staining。 . Spinal cord tissue and serum of TNF-a wasdetected separately by RT-PCR and ELISA. Spinal cord tissue of ICAM-1 wasdetected by RT-PCR and immunohistochemical staining.The result show during spinal cord I/R inject IL-10 using both s.c andi.v can elevate Tarlov score and improve spinal cord tissue injury comparewith single spinal cord I/R. and can inhibit serum and tissue of TNF-a andICAM-1 expression.The conclusion come from above investigate as follows:1. IL-10 has a certain expression during spinal cord I/R,and its expressionarise is more late,therefore do not inhibit injury come into being,but canalleviate spinal cord tissue injury along with I/R course. This maybe is abody protect responses against spinal cord I/R injury.2.During spinal cord I/R, inject IL-10 using both s.c and i.v way,can alleviate tissue injury and improve rat hindlimb movement function .which illuminate IL-10 has a protect role on spinal cord I/R.3. During spinal cord I/R, inject IL-10 using both s.c and i.v way,can inhibit TNF-a and ICAM-1 expression .That illuminate IL-10 maybeaffect spinal cord injury by inhibiting cytokines expression .4. The study of signification is that investigate systemic the effect ofIL-10 on spinal cord I/R,then offer a new strategy for spinal cord I/Rprevention and treatment.
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