The Effects Of Chronic Hypoxia And High Fat Diet On The Expression Of OxLDL And Lp-PLA₂ In Plasma Of Rabbit And Promoting Atherosclerosis Formation

Objective Establishment of chronic hypoxia model of New Zealand white rabbit, observation of chronic hypoxia and high fat died on the expression of oxLDL, Lp-PLA₂ in plasma and promoting formation of atherosclerosis.Method Twenty-four healthy New Zealand white rabbits were randomly divided into 4 groups, Normal control group, High fat group, Chronic hypoxia and Chronic hypoxia & high adipose diet group. There are 6 rabbits in each group. At the time of the 0w, the 4^th week, the 8^th week and the 12^th week, expression of oxLDL and Lp-PLA₂ in the plasma were measured by ELISA assay, And observe the pathological changes in their aortic arch and abdominal aorta with HE staining at the 12^th week. Determine whether the model is a success. All datas were analyzed by spss16.0, with repeated measure , one-way anove and bivariate.Results1. Plasma levels of oxLDL: (1) Comparison inner each group: Compared with 0^th week, At 4^th week, 8^th week and 12^th week, except the control group, plasma levels of oxLDL had certain degree influence on the other three groups(P<0.05), among which, At 8^th week increased most significantly in oxLDL concentrations(P<0.05). (2) Comparison within the group: Except the 0 th week, concentrations of oxLDL was significantly increased in other three groups compared with the control group (P<0.05); among which, At 4^th week, the levels of oxLDL increased more significantly in the chronic hypoxia group compared with the high-fat group (P<0.05), there was no statistical significance compared with chronic hypoxia group compared with high-fat group at 8^th week and 12^th week(P>0.05); At 4^th week, 8^th week and 12^th week, chronic hypoxia & high adipose diet group increased most significantly (P<0.05).2,Plasma levels of Lp-PLA₂: (1) Comparison inner each group: Compared with 0^th week, At 4^th week, there was no statistical significance to every group (P>0.05). Compared with 0^th week and 4^th week, At 8^th week and 12^th week, except the control group, plasma levels of Lp-PLA₂ had certain degree influence on the other three groups(P<0.05), among which, At 12^th week increased most significantly in Lp-PLA₂ concentrations(P<0.05). (2) Comparison within the group: Except the 0 th week and 4^th week, concentrations of Lp-PLA₂ was significantly increased in other three groups compared with the control group (P<0.05); among which, At 12^th week, the levels of Lp-PLA₂ increased more significantly in the chronic hypoxia group compared with the high-fat group (P<0.05); At 12^th week, chronic hypoxia & high adipose diet group increased most significantly compared with the other three groups(P<0.05).3 By repeated measurement analysis oxLDL and Lp-PLA₂ levels in plasma: what the repeated measurement time and the treatment groups influence significantly on plasma levels of oxLDL and Lp-PLA₂ were observde. There have interaction effect between repeated measurement time and the treatment groups(P<0.05). the plasma expression of oxLDL and Lp-PLA₂ were varied with different time and groups.4. Comparison of Weight: From the 0^th week to the 12^th week, we found that the weight of the control group, high fat group, chronic hypoxia and chronic hypoxia & high adipose diet group increased to varying degrees(P<0.05). But there is no statistic significance in the group within comparison of rabbit′s weight difference(P>0.05).5. Correlation analysis: Through correlation analysis, we found that the levels of oxLDL and Lp-PLA₂ were negatively correlated with weight (P>0.05).6. Observation in pathology intimates that,except the control group,the aortas and abdominal aorta of the other groups had varying degrees of local atherosclerosis pathological changes.Conclusions1 Chronic hypoxia and high-fat diet can promote the New Zealand white rabbits aortic arch and aorta atherosclerosis formation.2. Chronic hypoxia and high-fat diet can promote the plasma expression of oxLDL and Lp-PLA₂, there are varied with different time.3. There are significant increase of oxLDL and Lp-PLA₂ levels and promote atherosclerosis formation in Cooperation with chronic hypoxia and high-fat diet .