The Effect Of MACC1 On Invasion And Metastasis In Prostate Cancer Cell Lines (1Ee8/2B4)

【Background and Objective】In this study, we using siRNA to intervent highly- metastatic prostate cancer cell lines 1E8, compared with low-metastatic prostate cancer cell lines 2B4, studing the effects of MACC1 on invasion and metastasis in prostate cancer lines (1E8/2B4).【Methods】This study was designed to investigate the effect of MACC1 on metastasis and invasion in prostate cancer cells(1E8/2B4). Small interfering RNAs (siRNAs) were used to silence the expression of MACC1 in highly-metastatic prostate cancer cells 1E8, and the interfering effect was validated by western blot analysis and RT-PCR. Furthermore, we vertifyed the different expressions among normal prostatic tissue,prostatic hyperplasia,prostatic chronic inflammation,prostate tumors and metastasis. And detected the effects of invasion ande metastasis through scratch test,Transwell; used CCK-8 to observate the changes in cell proliferation; observated the imaging of cytoskeleton changes through confocal microscope.【Results】We validated the expression of MACC in the highly and lowly metastatic prostate cancer cell lines 1E8/2B4 through Western Blot and RT-PCR, the result is: the expression of MACC1 in 1E8 is stronger than 2B4; Through immnuohistochemistry in tissue microarray including normal prostate tissue,prostate hyperplasia,chronic inflammation of the prostate tissue,prostate cancer and metatasis, we found that there is a significantly difference of MACC1 in different prostate tissues. Furthermore, the expression of MACC1 is associated with stage and metastasis (P<0.05), but regardless of age and Gleason score (P>0.05); Observated MACC1 could significantly enhance the ability of cell movement of invasion and matastasis through scratch test and Transwell; Used CCK-8, we founded cell proliferation capacity diminished after interfering MACC1 in 1E8; Confocal showed that MACC1 induced the arrangement and polarization of cytoskeleton of prostate cancer.【Conclusion】In the highly-lowly metastatic prostate cancer cell lines 1E8/2B4, the expression of MACC1 in 1E8 is stronger than 2B4, prostate tissue microarray showed expression of MACC1 were statistical differences between prostate cancer and mormal prostate tissue, and it is associated with stage,metastasis, but regardless of age and Gleason scror ; Silenced the MACC1 in 1E8 by RNAi, than verificated that it increasts the invasion and metastasis through experiments of scratch test,transwell,confocal and CCK-8. The results showed that: anti-intervent 1E8 with the high expression of MACC1, reverse the the capability of invasion and metastasis. This will provide a new strategy for metastatic prediction, individualized treatment programs and even the reversion of tumor invasion. Objective To investigate the relationship between expression of Bub1 in different ovarian tissues and evaluated its clinical significance. Methods The expression of Bub1 were examined by immunohistochemical assay in 52 cases of ovarian cancer and 19 cases of Benign ovarian and 13 cases of Borderline ovarian lesions. And the significance of its expression was analysised with clinicopathologic parameter by X2 test. Results The positive rate of Bub1 protein in normal ovarian tissues, Benign ovarian, Borderline ovarian lesions and ovarian cancer was 23.5%, 5.3%, 76.9%, 94.2%, respectively. There were significant differences among the four ovarian tissues (P <0.05).The expression of Bub1 Protein was related to the staging, histological grade, lympahatic metestasis, recurrence and metastasis. Conclusion Abnormal expression of Bub1 is one of the mechanisms responsible for ovarian carcinogenesis. There may be some possible relationships between the initiation, progression and metastasis and ovarian cancer.