| Background and purpose Renal cell carcinoma is the most common malignancy of Urology.The incidence increased significantly in rencent years.The incidence in China after the bladder tumor. Renal clear cell carcinoma is the most common pathology type. At present,its diagnosis depends mainly on imaging, lack of the early diagnosis methods.One important reason is the lack of specific tumor markers,so find the ideal sensitivity and specificity for this tumor marker has a very important significance. CA IX is a newly discovered carbonic anhydrase isoforms of the family. In renal cell carcinoma have specific expression and in renal cell carcinoma diagnosis, prognosis and biological treatment has potential applications.Invasion and metastasis is an important indicator of malignancy. Invasion and metastasis of cancer is one of the reasons that treatment failure. Tumor invasion and metastasis is a process that multi-factors are involved and many steps to complete. Among them, the cell adhesion molecule on the connection and interaction between cells plays a key role in the inhibition of tumor invasion and metastasis. E-cadherin/catenin complex is an important cell adhesion molecule member of the tumor invasion and metastasis, the study CA IX and E-cadherin,β-catenin and two of them formed E-cadherin/catenin complexe can understand the relationship between CA IX in renal clear cell carcinoma invasion and metastasis implications. In this study, the expression of CA IX andβ-catenin ,E-cadherin are detected by immunohistochemistry in 62 cases of renal clear cell carcinoma. Investigate the role in the development of invasion and metastasis, especially for adhesion of the connection. Methods 62 cases of renal cell carcinoma specimens and 10 cases of normal kidney tissue are detected by Immunohistochemical Maxvision method.To gain CA IX, E-cadherin andβ-catenin protein expression and analysis of them with renal cell carcinoma histological grade, clinical stage . Compared to their invasive group and non-invasive group in the expression and to compare the relationship between the three. Results CA IX protein expression rate of renal clear cell carcinoma was 93.5%, while no expression in normal kidney tissue, and pathological grade and clinical stage was no significant difference (P>0.05); E-Cadherin,β- catenin abnormal expression rates were 67.7% (42/62) and 77.4% (48/62).The expression between E-Cadherin andβ- catenin have significant correlation (P<0.01); the abnormal expression of E-Cadherin,β-catenin protein were positively correlated with pathological grade and clinical stage (P<0.05); the abnormal expression of E-Cadherin,β-catenin protein between the invasive group and non-invasive group was significant difference (P<0.05); the abnormal expression of CA IX and E-Cadherin,β-catenin protein was positively correlated (P<0.05). Conclusions 1. CA IX protein is highly expressed in the RCCC, but not in normal renal tissue expression, suggesting that CA IX is expected to be an important specific tumor marker in RCC, particularly in RCCC; 2.The expression of E-cadherin andβ-catenin are correlated to the pathological grade and TNM stage. The abnormal expression in the invasive group and non-invasive group have differences, suggesting that the expression of them ,were correlated with the invasion and metastasis. 3. E-cadherin andβ-catenin expression was significantly correlated, the two together form E-cadherin/catenin complex, the complex and changing the surface of tumor cells and tumor cell adhesion is closely related to invasion and metastasis; Combined detection of E- cadherin,β-catenin will be conducive to more accurately determine the prognosis of patients and invasive of tumors. 4. the abnormal expression of CA IX was positively correlated with the E-cadherin andβ-catenin protein, suggesting that CA IX impact on the expression between E-cadherin andβ-catenin by influencing the microenvironment of tumor cells.While the abnormal expression of E-cadherin / catenin impact on the RCCC cell adhesion, thereby affecting the RCCC invasion and metastasis, but the impact of specific mechanisms remain to be confirmed by further studies. |
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