Gene Diagnosis Of Mig-7 Of Micrometastases In Lymph Nodes And Peripheral Blood In Patients With Lung Cancer And Its Clinical Significance

Objective To explore the clinical significance of gene diagnosis in detecting micrometastases in lung cancer by detecting Mig-7 mRNA expression in lymph nodes and peripheral blood.Methods The Mig-7 mRNA expression was detected in lesions, lymph nodes and peripheral blood from 30 lung cancer patients and 10 benign pulmonary lesion patients, and in the blood samples from 8 healthy volunteers by RT-PCR.Results The positive rates of Mig-7 mRNA expression were 77%, 51% and 27% in cancer tissues, lymph nodes and peripheral blood from lung cancer patients respectively. No Mig-7 mRNA was expressed in lesions, lymph nodes and peripheral blood from the patients with benign pulmonary lesions or in the peripheral blood from the volunteers.Conclusion The detection of Mig-7 mRNA in lymph nodes and peripheral blood from lung cancer patients by RT-PCR might be beneficial for diagnosing micrometastases early, identifying the stage, and providing important information in the selection of appropriate treatment strategies and for the evaluation of prognosis.