Function Research Of Th1/2,Th17 Cells And CD4~+ IL-10~+ Treg Cells Of Peripheral Blood In AR And DE

Objective :To investigate the Function of Th17cells and CD4+ IL-10+ Treg cells of peripheral blood in the patients of allergic rhinitis and Desensitization treatment effective.Methods:10 patients of severe perennial allergic rhinitis(AR)due to mite,10 healthy controls(HC)andDesensitization treatment effective(DE) were enrolled.peripheral blood mononuclear cells(PBMCs) were collected, respectively divided into three groups: ionomycin group(blank group) ,adding ionomycin 1ug/ml and monesin 2ug/l; mite group, adding ionomycin 1ug/ml; mite allergen nderp1 1ug and monesin 2ug/ml; PMA group , adding PMA 20ng/ml; ionomycin 1ug/ml and monesin 2ugl/ml. Cells were activated for 6h in carbondioxide cell incubator under 37℃,5% CO2.Then it was continuous to do intracellular and membrane staining with PBMCs and survey the percent of th17 cell in CD4+ cell by flow cytometric detector, and analyze the function of Th17 cells in the patients of AR and DE. Probe the percent of IL-10 cell in CD4 t cell. Results: The percents of Th17 cell in HC, Mite and PMA groups were inferior to corresponding AR group(P<0.05), also the percents of th17 cell in DE and Mite were be inferior to corresponding AR group(P<0.05). After the stimulated by mite, it was compared to blank AR decreased(P<0.05. After the stimulated by PMA, the percent of Th17 cell in HC, AR and DE were higher than blank group(P<0.05=. Otherwise the Treg cell of IL-10+ in HC, DE were lower than AR (P<0.05=. After stimulated by mite, AR was decreased to blank on the Treg cell of IL-10+ (P<0.05).Conclusions: It suggested that Th17 cells maybe had an influence of AR.PMA could increase the percent of Th cells secreting IL-17. It demonstrated the Treg cell secreting il-10 has a role of immunologic mechanism on allergic rhinitis. Objective :To investigate the Function of Th1/2cells of peripheral blood in the patients of allergic rhinitis and Desensitization treatment effective.Methods:10 patients of severe perennial allergic rhinitis(AR)due to mite , 10 healthy controls(HC)andDesensitization treatment effective(DE) were enrolled.peripheral blood mononuclear cells(PBMCs) were collected, respectively divided into three groups: ionomycin group(blank group) ,adding ionomycin 1ug/ml and monesin 2ug/ml; mite group, adding ionomycin 1ug/ml; mite allergen nderp1 1ug and monesin 2ug/ml; PMA group , adding PMA 20ng/ml; ionomycin 1ug/ml and monesin 2ugl/ml. Cells were activated for 6h in carbondioxide cell incubator under 37℃,5% CO2.Then it was continuous to do intracellular and membrane staining with PBMCs and survey the percent of th1/2 cell in CD4+ cell by flow cytometric detector, and analyze the function of Th1/2 cells in the patients of AR and DE. Probe the percent of th1/2 cell in CD4 t cell. Results: blank group,the percents of memory Th1 cell of HC was lower than that of AR, Mite groups were both decreased,but had no significantly. After the stimulated by PMA, the percent of Th1 cell in HC, AR and DE were higher than blank group(P<0.05). Blank group,the percents of memory Th2 cell of HC was higher than that of AR and DE, Mite groups were both had no significantly ,but had no significantly. After the stimulated by PMA, the percent of Th1 cell in HC, AR and DE were higher than blank group(P<0.05)Conclusions: It suggested that Th2 cells maybe had a special memory of AR.PMA could increase the percent of IFN-γand decrease the percent of IL-4.